品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / RayBiotech / RayBiotech/Human/Mouse AKT Pathway Phosphorylation Array C1/2 Sample Kit/AAH-AKT-1-2
商品描述
Antigen Information
Gene Symbols:
AKT1 | AKT1S1 | BAD | CDKN1B | EIF4EBP1 | GSK3A | GSK3B | MAPK1 | MAPK3 | MTOR| PDK1 | PRKAA1 | PTEN | RAF1 | RPS6 | RPS6KA1 | RPS6KA3 | RPS6KB1 | TP53 View more Hide
Assay Format
Pathway:
- Akt Signaling
- AMPK Signaling
- HER/ErbB Signaling
- HIF-1 alpha Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- mTOR Signaling
- PI3K-AKT Signaling
- TGF-beta Signaling
- Wnt/beta-Catenin Signaling
Number of Targets Detected:
18
Species Detected:
- Human
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473)(PKB (P-Ser473))
| AMPKa (P-Thr172)
| BAD (P-Ser112)
| 4E-BP1 (P-Thr36)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
|
GSK3a (P-Ser21)
| GSK3b (P-Ser9)
| mTOR (P-Ser2448)
| p27 (P-Thr198)
| P53 (P-Ser15)
|
P70S6K (P-Thr421/Ser424)
| PDK1 (P-Ser241)
| PRAS40 (P-Thr246)
| PTEN (P-Ser380)
| Raf-1 (Ser301)
|
RPS6 (P-Ser235/Ser236)
| RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Typical Data
Figure 1 - NIH3T3 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with PDGFβ for 10 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Figure 2 - 293 cells were grown to 80% confluency and then serum starved 24 hours. Cells were either untreated (bottom panel) or treated (top panel) with Insulin for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Yang Y, Wang J, Guo S, et al. Non-lethal sonodynamic therapy facilitates the M1-to-M2 transition in advanced atherosclerotic plaques via activating the ROS-AMPK-mTORC1-autophagy pathway. Redox Biol. 2020;32:101501. doi:10.1016/j.redox.2020.101501Species: MouseSample type: Cell culture
- Cai S, Yang Q, Cao Y, et al. PF4 antagonizes retinal neovascularization via inhibiting PRAS40 phosphorylation in a mouse model of oxygen-induced retinopathy. Biochim Biophys Acta Mol Basis Dis. 2020;1866(3):165604. doi:10.1016/j.bbadis.2019.165604Species: MouseSample type: Human vitreous fluid
- Qiao L, Hu S, Liu S, et al. microRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential.?J Clin Invest. 2019;129(6):2237-2250. Published 2019 Apr 29. doi:10.1172/JCI123135Species: HumanSample type: Cell Lysates
- Li M, Yang J, Zhang L, et al. A low-molecular-weight compound exerts anticancer activity against breast and lung cancers by disrupting EGFR/Eps8 complex formation.?J Exp Clin Cancer Res. 2019;38(1):211. Published 2019 May 22. doi:10.1186/s13046-019-1207-ySpecies: HumanSample type: Cell Lysates
- Sustova, Hana, et al. "Opposing effects of 25?hydroxy?and 1?, 25?dihydroxy?vitamin D3 on pro?cachectic cytokine?and cancer conditioned medium?induced atrophy in C2C12 myotubes."?Acta Physiologica?226.3 (2019): e13269.Species: MouseSample type: Cell Lysates
- Lu J, Liu QH, Wang F, et al. Exosomal miR-9 inhibits angiogenesis by targeting MDK and regulating PDK/AKT pathway in nasopharyngeal carcinoma.?J Exp Clin Cancer Res. 2018;37(1):147. Published 2018 Jul 13. doi:10.1186/s13046-018-0814-3Species: HumanSample type: Cell lysates
- Chiurchiu V., Rapino C., Talamonti E., et al. Anandamide Suppresses Proinflammatory T Cell Responses In Vitro through Type-1 Cannabinoid Receptor-Mediated mTOR Inhibition in Human Keratinocytes. J Immunol. 2016 Nov 1;197(9):3545-3553. doi: 10.4049/jimmunol.1500546Species: HumanSample type: Cell Lysate
- Chiurchiù, Valerio, et al. "Anandamide suppresses proinflammatory T cell responses in vitro through type-1 cannabinoid receptor?mediated mTOR inhibition in human keratinocytes."?The Journal of Immunology?197.9 (2016): 3545-3553.Species: HumanSample type: Cell Lysates
Cleveland Clinic09/14/2018, 01:13 PM
04/06/2018, 03:37 PM
Manual
SDS
Analysis Tool
