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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
CCL1 | CCL11 | CCL12 | CCL17 | CCL2 | CCL24 | CCL3 | CCL5 | CCL9 | CSF1| CSF2 | CSF3 | CXCL1 | CXCL13 | CXCL5 | CXCL9 | FASLG | ICAM1 | IFNG | IL10 | IL12A | IL13 | IL15 | IL17A | IL1A | IL1B | IL2 | IL21 | IL3 | IL4 | IL5 | IL6 | IL7 | LEP | PF4 | TIMP1 | TNF | TNFRSF1A | TNFRSF1B | TNFSF8 View more Hide
Assay Format
Number of Targets Detected:
40
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
BLC(CXCL13)
| CD30 Ligand(TNFSF8)
| Eotaxin-1(CCL11)
| Eotaxin-2(MPIF-2/CCL24)
| Fas Ligand(TNFSF6)
|
GCSF
| GM-CSF
| I-309(TCA-3/CCL1)
| ICAM-1(CD54)
| IFN-gamma
|
IL-1 alpha(IL-1 F1)
| IL-1 beta(IL-1 F2)
| IL-10
| IL-12 p70
| IL-13
|
IL-15
| IL-17A
| IL-2
| IL-21
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-7
| KC(CXCL1)
|
Leptin
| LIX
| MCP-1(CCL2)
| MCP-5
| M-CSF
|
MIG(CXCL9)
| MIP-1 alpha(CCL3)
| MIP-1 gamma
| Platelet Factor 4(CXCL4)
| RANTES(CCL5)
|
TARC(CCL17)
| TIMP-1
| TNF alpha
| TNF RI(TNFRSF1A)
| TNF RII(TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Shi Y., Zhang L., Pu H., et al. Rapid endothelial cytoskeletal reorganization enables early blood-brain barrier disruption and long-term ischaemic reperfusion brain injury. Nat Commun. 2016 Jan 27;7:10523. doi: 10.1038/ncomms10523.Species: MouseSample type: Tissue Lysate
- Brod S. Ingested (oral) anti-IL-12/23 inhibits EAE. J Neurol Sci. 2016 Feb 15;361:19-25. doi: 10.1016/j.jns.2015.12.011. Species: MouseSample type: Conditioned Media
- Dou Y., Zhang X., Xu X., et al. Multiple noncovalent interactions mediated one-pot therapeutic assemblies for the effective treatment of atherosclerosis. J. Mater. Chem. B, 2015,3, 7355-7365, DOI: 10.1039/C5TB01474CSpecies: MouseSample type: Serum
- Sheedy, Frederick J., et al. "CD36 coordinates NLRP3 inflammasome activation by facilitating intracellular nucleation of soluble ligands into particulate ligands in sterile inflammation."?Nature immunology?14.8 (2013): 812.Species: MouseSample type: Serum
- Morin, C., Roumegous, A., Carpentier, G., Barbier-Chassefiere, V., Garrigue-Antar, L., Caredda, S., & Courty, J. (2012). Modulation of inflammation by Cicaderma ointment accelerates skin wound healing.?Journal of Pharmacology and Experimental Therapeutics,?343(1), 115-124.Species: MouseSample type: Tissue Lysate
- Zeng Q., Chen W. The functional behavior of a macrophage/fibroblast co-culture model derived from normal and diabetic mice with a marine gelatineoxidized alginate hydrogel. Biomaterials 31 (2010) 5772-5781Species: MouseSample type: Conditioned Media
- Zeng Q., et al. The functional behavior of a macrophage/fibroblast co-culture model derived from normal and diabetic mice with a marine gelatin-oxidized alginate hydrogel. Biomaterials. 2010 Aug;31(22):5772-81. doi: 10.1016/j.biomaterials.2010.04.022.Species: MouseSample type:
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