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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
AGRP | ALCAM | ANG | ANGPT1 | AREG | AXL | BDNF | BMP4 | BMP5 | BMP7| BTC | CCL1 | CCL11 | CCL13 | CCL14 | CCL15 | CCL16 | CCL17 | CCL18 | CCL19 | CCL2 | CCL20 | CCL21 | CCL22 | CCL23 | CCL24 | CCL25 | CCL26 | CCL27 | CCL28 | CCL3 | CCL4 | CCL5 | CCL7 | CCL8 | CD14 | CD40 | CD40LG | CD80 | CDH1 | CEACAM1 | CNTN2 | CSF1 | CSF1R | CSF2 | CSF3 | CTSS | CXCL1 | CXCL10 | CXCL11 | CXCL12 | CXCL13 | CXCL16 | CXCL5 | CXCL6 | CXCL8 | CXCL9 | DKK1 | EDA2R | EGF | EGFR | ENG | EPCAM | ERBB3 | FAS | FASLG | FCGR2B | FGF2 | FGF4 | FGF7 | FIGF | FLT1 | FLT3LG | FLT4 | FST | GDF15 | GDNF | GH1 | HAVCR1 | HBEGF | HGF | ICAM1 | ICAM2 | ICAM3 | IFNG | IFNL1 | IFNL2 | IGF1 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IGFBP6 | IL10 | IL10RB | IL11 | IL12A | IL12B | IL13 | IL13RA1 | IL13RA2 | IL15 | IL16 | IL17A | IL17B | IL17F | IL17RA | IL18BP | IL1A | IL1B | IL1R1 | IL1RL1 | IL1RN | IL2 | IL21R | IL23A | IL2RA | IL2RB | IL2RG | IL31 | IL4 | IL5 | IL6 | IL6R | IL6ST | IL7 | IL9 | INHBA | INS | KDR | KIT | KITLG | LCN2 | LGALS7 | LIF | LTA | LYVE1 | MICA | MICB | MIF | MOK | MST1 | NGF | NGFR | NRCAM | NRG1 | NTF3 | NTF4 | PARN | PDGFA | PDGFB | PDGFRB | PECAM1 | PF4 | PGF | PI3 | PLAUR | PLG | PPBP | PROK1 | RETN | SCARB2 | SELE | SELL | SERPINE1 | SHH | SIGLEC5 | SPP1 | TDGF1 | TEK | TGFA | TGFB1 | TGFB2 | TGFB3 | THPO | TIMP1 | TIMP2 | TNF | TNFRSF10C | TNFRSF10D | TNFRSF11B | TNFRSF14 | TNFRSF17 | TNFRSF18 | TNFRSF1A | TNFRSF1B | TNFRSF21 | TNFRSF8 | TNFRSF9 | TNFSF14 | TREM1 | TSLP | TYRO3 | VCAM1 | VEGFA | VEGFC | XCL1 View more Hide
Assay Format
Number of Targets Detected:
200
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 250 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), Activin A, AgRP, ALCAM (CD166), Amphiregulin, Angiogenin, Angiopoietin-1, Angiostatin, AXL, BCMA, BDNF, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-7, Cathepsin S, CCL28 (MEC), CD14, CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD80 (B7-1), CEACAM-1, Contactin-2 (TAG1), Cripto-1, CTACK (CCL27), CXCL16, DAN, DKK-1, DR6 (TNFRSF21), Dtk, E-Cadherin, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB3, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), FGF-4, FGF-7 (KGF), Flt-3 Ligand, Follistatin, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GM-CSF, gp130 (sgp130), GRO alpha/beta/gamma, Growth Hormone, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R4 (ST2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-16, IL-17 RA, IL-17A, IL-17B, IL-17F, IL-18 BP alpha, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-21 R, IL-23, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-31, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1 (TGF beta 1), LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NrCAM, NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, Platelet Factor 4 (CXCL4), PLGF, RAGE, RANTES (CCL5), Resistin, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog N-Terminal (Shh-N), TARC (CCL17), TECK (CCL25), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin (TPO), Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trappin-2, TREM-1, TROP1 (EpCAM), TSLP, uPAR, VCAM-1 (CD106), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, XEDARApplication Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Human Cytokine Array Q4000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, ore the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
"- Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.Species: HumanSample type: Serum
- Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.Species: HumanSample type: Vitreous Fluid
- Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.Species: HumanSample type: Vitreous Fluid
- Martinaud C., Desterke C., Konopacki J., et al. Osteogenic Potential of Mesenchymal Stromal Cells Contributes to Primary Myelofibrosis. Cancer Res. 2015 Nov 15;75(22):4753-65. doi: 10.1158/0008-5472.CAN-14-3696Species: HumanSample type: Conditioned Media
01/25/2018, 01:50 PM
Manual
SDS
Analysis Tool (QAH-INF-3)
Analysis Tool (QAH-GF-1)
Analysis Tool (QAH-CHE-1)
Analysis Tool (QAH-REC-1)
Analysis Tool (QAH-CYT-4)
