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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
Antigen Information
Accession Number:
- P60022
Gene ID:
- 1672
Gene Symbol:
- DEFB1 BD1 HBD1
Protein Name & Synonyms:
Beta-defensin 1 (BD-1) (hBD-1) (Defensin, beta 1)
Assay Format
Detection Range:
0.1-1,000 ng/ml
Recommended Dilution (serum & plasma):
Human: 2x / Mouse: 2x / Rat: 2x
Sensitivity:
0.9 ng/ml
Specificity:
This EIA kit is designed to detect human, mouse, and rat Beta-defensin 1
Number of Targets Detected:
1
Species Detected:
- Human
- Mouse
- Rat
Compatible Sample Types:
- Cell Culture Supernatants
- Serum
Design Principle:
- Competition-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Introduction
Beta-defensin 1 (BD-1) is a 36 amino acid peptide that has bactericidal activity in humans. It is encoded by the DEFB1 gene, and belongs to a family of microbicidal and cytotoxic peptides made by neutrophils. BD-1 is believed to function in the antimicrobial defense of the urogenital and respiratory tracts.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Typical Data
Standard Curve
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
Temple University10/17/2018, 11:51 AM
Manual
SDS
