Proteinase K is an endolytic protease that cleaves peptide bonds atthe carboxylic sides of aliphatic, aromatic or hydrophobic amino acids.Proteinase K is classified as a serine protease. The smallest peptide tobe hydrolysed is a tetrapeptide.
DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biologicalsamples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR ReagentProtocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for~24 hrs.
Other applications: Proteinase K digests nativeproteins very effectively. It can therefore be used to remove DNases andRNases when isolating DNA and RNA from tissues or cells.
Usage Conditions:
• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At high temperatures—optimum digestion temperature is 65°C
Unit Definition: One unit of the enzymeliberates folin-positive amino acids and peptides corresponding to 1μmoltyrosine in 1min at 37°C using denatured hemoglobin as substrate.
Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases
ViagenBiotechInc.是为生命科学研究提供创新DNA提取技术和产品的领先提供商。ViagenDirectPCR®DNA提取系统是一种单管系统，用于快速从小鼠尾巴，耳件，卵黄囊和培养细胞中制备DNA。由ViagenBiotechInc.的科学家开发的正在申请专利的组件使所得的DNA提取物与基因组PCR兼容用于基因分型。生物样品的粗提物与许多分子生物学级反应（例如聚合酶链反应（PCR））不兼容，部分原因是粗提物中含有抑制剂。DirectPCR试剂不仅可以介导生物样品的快速裂解，而且还包含能有效抑制粗裂解物对PCR扩增的抑制活性的抑制剂，同时最大程度地保持释放的基因组DNA的完整性。该程序完全消除了任何溶液转移或管子打开步骤，从而节省了大量时间和精力。DirectPCR试剂每条尾巴的成本仅为26美分，可在任何用量的情况下节省成本。
与其他领先公司相比，我们还提供最实惠的DNA标记。我们将通过积极寻求与学术机构和行业创新者的研究和业务合作伙伴关系来开发新的研究工具，从而继续扩大我们的能力。我们的产品销往学术研究市场，生物技术公司和制药公司。通过由30多个国际分销商组成的全球网络，我们为全球生命科学研究科学家网络提供优质的产品和服务。
ViagenBiotechInc.isaleADIngproviderofinnovativeDNAextractiontechnologiesandproductsforlifescienceresearch. ViagenDirectPCR® DNAExtractionSystemisasingle-tubesystemforrapidpreparationofDNAfrommousetails,earpieces,yolksac,andculturecells. Thepatent-pendingcomponentsdevelopedbyscientistsatViagenBiotechInc.allowtheresultingDNAextractstobecompatIBLewithgenomicPCRforgenotyping.CrudeextractsofBIOLOGicalsamplesarenotcompatiblewithmanymolecularbiology-gradereactionssuchaspolymerasechainreaction(PCR),inpartduetoinhibitorscontainedincrudeextracts. TheDirectPCRreagentsnotonlymediatetherapidlysisofbiologicalsamplesbutalsocontaininhibitorsthateffectivelysuppresstheinhibitoryactivitiesofcrudelysatesforPCRamplification,whilemaximallymaintainingtheintegrityofreleasedgenomicDNA.Theprocedurecompletelyeliminatesanysolutiontransferortube-openingsteps,savingasubstantialamountoftimeandeffort.DirectPCRreagentscostonly26centspertail,providingsavingsatanyusagevolume.
WealsoofferthemostaffordableDNAMarkerscomparedtootherleadingcompanies.WewillcontinuetoexpandourcapABIlitiesbyactivelyseekingresearchandbusinesspartnershipwithbothacademicinstitutionsandindustryinnovatorstodevelopnewresearchtools. Ourproductsaresoldtoacademicresearchmarkets,biotechnologycompanies,andpharmaceuticalcompanies.Throughaglobalnetworkofmorethan30internationaldistributors,weofferourqualityproductsandservicestoaworldwidenetworkofresearchscientistsinlifescience.
