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4000-520-616 / 18915418616
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0512-67156496
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商品描述
SirT1 (phospho-Ser27) rabbit pAb
- Catalog No.：YP1495
- Applications：WB
- Reactivity：Human
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- SIRT1 SIR2L1
- Protein Name：
- SirT1 (Ser27)
- Human Gene Id：
- 23411
- Human Swiss Prot No：
- Q96EB6
- Mouse Gene Id：
- 93759
- Mouse Swiss Prot No：
- Q923E4
- Immunogen：
- Synthesized phosho peptide around human SirT1 (Ser27)
- Specificity：
- This antibody detects endogenous levels ofHuman SirT1 (phospho-Ser27)
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- WB 1:1000-2000
- Purification：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- NAD-dependent protein deacetylase sirtuin-1 (hSIRT1) (EC 3.5.1.-) (Regulatory protein SIR2 homolog 1) (SIR2-like protein 1) (hSIR2) [Cleaved into: SirtT1 75 kDa fragment (75SirT1)]
- Observed Band(KD)：
- 85-110
- Background：
- sirtuin 1(SIRT1)Homo sapiensThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],
- Function：
- catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3,5,4"-trihydroxy-trans-stilbene), butein (3,4,2",4"-tetrahydroxychalcone), piceatannol (3,5,3",4"-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2",4"-trihydroxychalcone), fisetin (3,7,3",4"-tetrahydroxyflavone) and quercetin (3,5,7,3",4"-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity.,function:NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates "Lys-382" of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1
- Subcellular Location：
- nuclear chromatin,nucleus,nuclear envelope,nuclear inner membrane,nucleoplasm,chromatin silencing complex,nuclear euchromatin,nuclear heterochromatin,nucleolus,cytoplasm,mitochondrion,PML body,rDNA heterochromatin,
- Expression：
- Brain,Epithelium,Prostate,Testis,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- June 19-2018
- FLOW CYTOMEYRT PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
