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0512-67156496
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商品描述
Rad18 (phospho-Ser403)rabbit pAb
- Catalog No.：YP1458
- Applications：WB
- Reactivity：Human
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name：
- RAD18 RNF73
- Protein Name：
- Rad18 (Ser403)
- Human Gene Id：
- 56852
- Human Swiss Prot No：
- Q9NS91
- Mouse Gene Id：
- 58186
- Mouse Swiss Prot No：
- Q9QXK2
- Immunogen：
- Synthesized phosho peptide around human Rad18 (Ser403)
- Specificity：
- This antibody detects endogenous levels ofHuman Rad18 (phospho-Ser403)
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- WB 1:1000-2000
- Purification：
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- E3 ubiquitin-protein ligase RAD18 (EC 6.3.2.-) (Postreplication repair protein RAD18) (hHR18) (hRAD18) (RING finger protein 73)
- Observed Band(KD)：
- 56
- Background：
- RAD18, E3 ubiquitin protein ligase(RAD18) Homo sapiensThe protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif.Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008],
- Function：
- function:E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on "Lys-164". Has ssDNA binding activity.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the RAD18 family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SAP domain.,subunit:Interacts with UBE2A and UBE2B. Interacts with SHPRH.,
- Subcellular Location：
- nucleus,nucleoplasm,replication fork,cytoplasm,centrosome,site of double-strand break,nuclear inclusion body,Rad6-Rad18 complex,
- Expression：
- Epithelium,Liver,Placenta,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- June 19-2018
- FLOW CYTOMEYRT PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
