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商品描述
WST-8
- Catalog No.：MC1160
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- CasNo：
- 193149-74-5
- MolecularFormula：
- C20H13N6NaO11S2
- Purity：
- >98%
- Target：
- See reference
- IC50：
- No data
- In Vitro：
- The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].
- In Vivo：
- Fields：
- WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.
- Specificity：
- Target: See reference. Fields: WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.
- Dilution：
- Concentration：
- >98%
- Storage Stability：
- 2 years -20°C Powder, 2 weeks4°C in DMSO,6 months-80°C in DMSO
- Other Name：
- WST8,WST 8
- MolecularWeight(Da)：
- 600.47
- References：
- [1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.
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