**Please contact us for a custom quote or to discuss process of two part screening**

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The Whole-Cell Methylerythritol Phosphate (MEP) Pathway-Selective Antibacterial Screening Assay, described in [1], utilizes a genetically engineered strain of Salmonella typhimurium (CT31-7d) that can utilize either the MEP or the mevalonate (MVA)pathways in order to produce isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the two essential precursors for all isoprenoids. In the absence of arabinose and MVA, the MEP pathway is utilized for growth. When supplemented with arabinose and MVA, the engineered MVA pathway is activated. Compounds inhibiting MEP pathway growth with no effect on MVA pathway growth are selective for the MEP pathway. Validated using known MEP pathway inhibitors and other antibacterials, the screening platform is more sensitive to MEP pathway inhibitors than wild-type S. typhimurium. Antibacterials targeting other pathways demonstrate similar minimum inhibitory concentrations (MICs) for both MEP and MVA pathway growth.

The Whole-Cell MEP Pathway-Selective Antibacterial Screening Assay is performed in a 96 well plate and consists of two stages:1. Primary Antibacterial Screen: This screen uses our engineered cell line under growth conditions with only the MEP pathway active to identify initial antibacterial “hits”. Compounds are added in a 96-well plate and screened in duplicate. This screen tests a single concentration (usually 100 µg/mL) or a user determined amount against each pathway. We are able to screen up to 80 compounds per plate in duplicate.2. Secondary MEP Pathway-Selectivity Screen: Antibacterial “hits” are subjected to dose response analysis evaluating MEP growth in one plate and MVA in a second to determine MICs for both growth conditions. Using CT31-7d and different growth conditions allow us to determine if the antibacterial hit displays specificity toward the MEP pathway. Dose-response experiments are performed on 4 compounds per plate in duplicate for the secondary screen. Fosmidomycin, a known MEP pathway inhibitor, is included in both screens as a positive control. The concentration used (33 μM) is insufficient to kill wild-type bacteria, but is effective against CT31-7d due to heightened MEP pathway sensitivity.

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Echelon Biosciences成立于1997年10月3日，总部坐落于在美国犹他州。从1997到2004，EBI主要用于政府的补助金以及产品的销售收入来资助其研究工作。2005年初，EBI的依特钠Zentaris购买 （NAS：AEZS）的MEP抗感染，PI3激酶小分子程序，目录业务，与科学专业。2007年末，前沿科学公司（FSI）在洛根，犹他州购买了EBI， 专业从事卟啉和硼化学。自公司成立以来以来，EBI保留了开发研究试剂脂质新的检测方法。不断开拓**，从而推动药物研究的新发型。 Echelon Biosciences是美国杰出免疫学产品供应商，专业提供各种抗体、分析检测试剂盒、抑制剂等产品，尤其擅长以细胞信号、脂类代谢和标记物研究为主的相关产品。为研究治疗诸如癌症、糖尿病、炎症、感染和心脑血管疾病专家提供**有效工具。 Echelon Biosciences产品线 Lipid Kinases & Phosphatases 脂质激酶和磷酸 Lipid-Protein Interaction Tools 脂蛋白质相互作用工具 Lipid-Specific Assay Kits 脂质特定检测试剂盒 Lipid-Specific Antibodies & Binding Proteins 脂质特定抗体和结合蛋白 Hyaluronic Acid & Collagen IV Assay Kits 透明质酸和IV型胶原检测试剂盒 Synthetic Inositol phosphates, Lipids, & Phospholipids 合成的磷酸肌醇，脂和磷脂 PI3-Kinase PI3-激酶 Echelon Biosciences可提供PI3-Kinase 实验解决全套方案，从分析服务到实验用抗体、酶和抑制剂等，**满足科研需求。 专业系列 产品编号 特色产品 Kits 试剂盒 K-1000 PI3-K ELISA Kit  K-1000S PI3-K ELISA: Pico Assay K-1100 PI3-K Fluorescence Polarization Kit K-1300 PI3-K AlphaScreen®  K-2700S TRueFRET PI3-K Activity Assay K-3000 Class III PI3-K ELISA Kit Antibodies 抗体 Z-R015 Anti-huVps34 (Class III PI3-Kinase) Antibody for IP Z-R016 Anti-huVps34 (Class III PI3-Kinase) Antibody for WB Inhibitors 抑制剂 B-0021 Resveratrol (Class IA PI3-K Inhibitor) B-0222 Wortmannin (PI3-Kinase Inhibitor) B-0294 PI3-Kinase Inhibitor (LY294002) B-0301 PI3-Kinase Gamma Inhibitor 1 (AS605240) B-0302 PI3-Kinase Gamma Inhibitor 2 (AS604850 B-0303 PI3-Kinase Alpha Inhibitor 1 (PI-103) B-0304 PI3-Kinase Alpha Inhibitor 2 Enzymes 酶  P27-18H PI3-K (p-110 alpha/p85 alpha) Active Enzyme  P28-10H PI3-K (p-110 beta/p85 alpha) Active Enzyme Lysolipids 血溶性脂类 Lysolipid Pathway                                            Synthetic Lysophosphatidic Acid (LPA) ◆ Inhibitors 抑制剂系列                                  ◆ Labeled 标记物系列 ◆ Ligands 配体系列                                        ◆ Ligands 配体系列 ◆ Analogs 类似物系列                                    ◆ Saturated 底物系列                                                                          ◆ Unsaturated 不饱和系列  专业系列 产品标号 特色产品 Kits试剂盒  K-2800B Lysophosphatidic Acid Competitive ELISA Kit K-4100 Autotaxin Activity Assay K-4200 Autotaxin Inhibitor Screening Kit Enzymes酶 E-4000 Autotaxin Enzyme Substrate底物 L-2000 FS-3 LysoPLD/Autotaxin Substrate Inhibitors抑制剂 L-7416 LysoPLD/Autotaxin Inhibitor Sphingolipids 鞘脂类 Synthetic Sphingolipids ◆ Sphingosine 鞘氨醇                           ◆ Ceramide 神经酰胺 ◆ Sphingosine 1 Phosphate (S1P ) 鞘氨醇磷酸酯     ◆ Sphingomyelin 鞘磷脂 ◆ FTY720P  专业系列 产品编号 特色产品 Kits 试剂盒 K-1900 Sphingosine 1 Phosphate ELISA Kit (S1P) K-3500 Sphingosine Kinase 1 Activity Assay K-1800 Sphingomyelinase Assay Kit K-3200 Acid Sphingomyelinase Assay Kit Enzymes 酶 E-K068 Sphingosine Kinase 1, active E-K069 Sphingosine Kinase 2, active Inhibitors 抑制剂 B-0024 Sphingosine Kinase Inhibitor   产品编号 特色产品 S-400B Sphingomyelin Biotin  P-B0SM Sphingomyelin Beads S-6100 Sphingosine Beads S-6110 Sphingosine 1 Phosphate Beads  P-B00Ss Sphingo Bead Sample Pack  G-SM01 Sphingomyelin Grip (Equinatoxin) Autotaxin(ATX) 自分泌运动因子 Echelon Biosciences是世界上唯壹完整**提供Autotaxin(ATX) 和Autotaxin(ATX) 相关试剂的供应商。 产品编号 特色产品 K-4100 Autotaxin Activity Assay Kit K-4200 Autotaxin Inhibitor Screening Kit  K-2800B Lysophosphatidic Acid Competitive ELISA Kit L-2000 FS-3 (LysoPLD / Autotaxin Substrate) L-7416 BrP-LPA Autotaxin Inhibitor E-4000 Autotaxin Enzyme