MutaGEL r-Vitamin D3 PCR Assay
MutaGEL r-Vitamin D3 PCR Assay manufactured in Germany by Immundiagnostik
Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Controls Included
Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the “VD3R positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).
Assay Principle
The MutaGEL® r-vitamin D3 PCR Assay contains a set of primers for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of varying genotypes (start codon polymorphism) is characterized by subsequent specific restriction enzyme digestion. The rare variant (f, pathogenic) possesses a restriction site for the specific endonuclease, whereas the major variant (F, protective) does not. The amplified products obtained from the pathogenic DNA variant will, therefore, be cut into two fragments, whereas the protective DNA variant will not be cut. The identification of the existing genotype from the patient is done by subsequent analysis of the DNA fragments by gel-electrophoretic methods (Dr. Essrich, Biologisches Labor, Denzlingen).
Related Products
MutaGEL r-Calcitonin PCR Assay
MutaGEL® Parodontitis PCR Assay
MutaGEL® Lactase (AS) PCR Assay
Learn more about Vitamin D at Eagle Biosciences Biomarker Spotlight page dedicated to Vitamin D here:
Vitamin D Biomarker Spotlight
EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。
