25-OH Vitamin D HPLC Assay

The 25-OH Vitamin D HPLC Assay is For Research Use Only

Size: 100 tests
Incubation Time: 15 minutes
Sample Type: Plasma, Serum
Sample Size: 400 µL
Controls Included

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Reference Values (Normal): 
Summer    50-300 nmol/l (20-120 µg/l)
Winter       25-150 nmol/l (10-60 µg/l)

It is suggested that each laboratory establish its own normal ranges

Product Developed and Manufactured in Germany
Product Support in the USA

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Assay Background

Vitamin D is one of the most important essential bioregulators of the Ca2+- and phosphate metabolism in higher animals. Together with the peptide hormones parathormone and calcitonin it is responsible to obtain the Ca2+- and phosphate homoeostasis.  The most important physiological functions of vitamin D are the stimulation of the intestinal resorption of Ca2+ and phosphate and their incorporation in the bone matrix.

D-vitamins or calciferols arise from provitamins by the UV radiation of sunlight catalyzed splitting of the B-ring of the steran backbone. In the skin, formed vitamin D3 is bound to a vitamin D binding-protein in the plasma, transported into the liver and hydroxylated in position 25 to form 25-OH vitamin D3. Another hydroxylation is performed in the kidney to yield 1,25 (OH)2 vitamin D3 and 24,25 (OH)2 vitamin D3.   1,25 (OH)2 vitamin D3 is the metabolic most active form of vitamin D.  Nevertheless, more than 95% of 25-OH vitamin D is 25-OH vitamin D3.  A lack of vitamin D leads in growing humans and animals can lead to rickets, hypocalcaemia and secondary hyperparathyroidism and in adults to osteomalacia.

The Eagle Biosciences 25-OH Vitamin D3 HPLC Assay kit makes it possible to determine the vitamin in an easy, fast and precise method. The kit includes all reagents in ready to use form for preparation and separation of the samples with exception of the column (IC3401rp) and the controls (IC3401ko). Both can be supplied by Eagle Biosciences.  Beside the complete test kit, it is possible to order all components separately. Please request our single component price list.

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Related Products

25-OH Vitamin D HPLC Assay Control Set
25-OH Vitamin D HPLC Assay Column
25-OH Vitamin D ELISA Assay Kit

EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。