Porphyrins HPLC Assay Column

The Porphyrins HPLC Assay Column is For Research Use Only

Each column is can be utilized for approximately 500-1000 samples depending on column care and sample type. 

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Assay Background

Hemoglobin, myoglobin and the cytochromes contain the heme structure as active site. The porphyrins are precursors of the heme molecules. They play an important role in the oxygen metabolism. The heme synthesis is located mainly in the erythrocyte cells. Glycin and succinyl-CoA are condensed to δ-aminolävulinic acid. Two molecules of δ-aminolävulinic are then condensed to porphobilinogen. The porphyrin ring is built up by four porphobilinogen molecules.

An increase of porphyrins caused by a defect in the synthesis is described as porphyria. The different porphyries are determined by the characteristic pattern of porphyrins in urine.   In autosomal dominant acute porphyrin disorders uro-, copro-, penta- und tricarboxyporphyrin are increased. In chronic porphyries including porphyria cutanea tarda the concentration of uro- and heptaporphyrin are increased. In chronic lead intoxication the coproporhyrins are slightly increased, whereas an acute lead intoxication shows extremely high excretion of porphyrins in urine.

The Eagle Biosciences Porphyrins HPLC Assay Kit makes it possible to determine them in an easy, fast and precise way. The Porphyrins HPLC Assay Kit includes all reagents in ready to use form for preparation and separation of the samples with exception of the columns (IC2601rp) and the controls (IC2601ko).   Both can be supplied by Eagle Biosciences.  Beside the complete test kit it is possible to order all components separately. Please request our single component price list.

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EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。