easYmer HLA-B*35:01 MHC Tetramers Kit

The easYmer HLA-B*35:01 MHC Tetramers Kit is for Research Use Only

Materials Included in Kit:

• easYmer: HLA-B*35:01 – peptide receptive, biotinylated in Tris/Maleate pH 7 with 30% Glycerol
• Folding Buffer: Tris/Maleate pH 7
• Peptide: YF NS2A4–13 (HAVPFGLVSM), a known HLA-B*35:01 binder
  Positive control for evaluation analysis of peptide-HLA folding

Available Sizes:

• 50 Tests (Standard Size)
• 150 Tests
• 500 Tests

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Key Benefits of easYmer® MHC Tetramers

• Ready-to-use
• One step loading
• Completely flexible and customizable*
• Biotinylated
• No special equipment needed
• Long shelf-life

*For Custom Tetramer Production, please Contact Us for more information

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Scientific Principle

This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß₂-microglobulin (ß₂m? light chain subunit of recombinant HLA Class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß₂m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

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Related Products

easYmer HLA-C*07:02 MHC Tetramers Kit
easYmer HLA-B*44:02 MHC Tetramers Kit
easYmer HLA-A*11:01 MHC Tetramers Kit

Product Developed and Manufactured by immunAware

EagleBio的5α-雄甾烷-3α，17β-二醇葡糖醛酸化物是C19类固醇，缩写为3α-DiolG，5α二醇G或简称为α二醇G。它主要作为睾丸激素和二氢睾丸激素（DHT）的代谢产物生产。它主要在目标外围组织（例如皮肤）中产生，尤其是在毛囊周围。大量的3α-DiolG刺激会导致过多的头发形成，特别是在女性通常不存在头发的地方。近年来，在研究患有特发性多毛症的妇女的临床研究人员中，人们对这种类固醇的测量越来越感兴趣。已知是3α-DiolG前体的类固醇包括脱氢表雄酮（DHEA），硫酸脱氢表雄酮（DHEAS），二氢睾丸酮（DHT），雄烯二酮和睾丸激素。已显示只有3α-DiolG随多毛症增加而随治疗减少。这种相关性在多囊卵巢综合征（PCO）患者中也得到了证明。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。3α-DiolG的测定因此被证明是多种方式的有用指标，包括监测特发性多毛症和PCO妇女的治疗进展。此外，接受环孢霉素A治疗的糖尿病患者（无论男女）均显示3α-DiolG水平升高，这种副作用导致以前无毛的区域出现了头发。