Ochratoxin Immunoaffinity HPLC Column

The Ochratoxin Immunoaffinity HPLC Column is For Research Use Only

Size: 3ml, 10 columns

Assay Principle

These instructions for Ochratoxin determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (Immunoaffinity Chromotography (IAC)) in combination with quantification with HPLC (High Performance Liquid Chromotography).  Accepted laboratory extraction methods could be maintained. If the criteria of organic solvent tolerance, elution process of analyte, and working range of column are followed you will see full performance with the IAC column.  If problematic matrices are applied, many pretreatment methods of Ochratoxin determination in food and feed will show low sensitivity because of interfering substances.  This method of content determination of ochratoxins combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate eluate with the additional step of purification by HPLC column.

Please notice that this instruction focuses on the handling with the IAC column. For the commodity extraction step please note that a literature method is provided. The given apparatus (e.g. HPLC system) might serve as example among other possibilities.

Extraction (Literature method given):
Assuming that 25g sample are extracted by a total of 100ml methanol/water (80/20 v/v). If organic solvent proportion is varied the dilution of extract with PBS should be adapted accordingly in the enrichment step. On the other hand, if proportion of sample quantity and volume of extraction solvent is altered, calculation of gram equivalents must be corrected.  Grains could be prepared by the literature method of Olsson et al. For milk as an example of a problematic matrix, method of Zimmerli et al. could be applied.

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Product Developed and Manufactured in Germany by BioTeZ Berlin-Buch GmbH

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