15-Isoprostane F2t ELISA Assay

The 15-Isoprostane F2t ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.05 ng/ml
Dynamic Range: 0.05 – 100 ng/ml
Incubation Time: 2.5 hour
Sample Type: Biological Fluids
Sample Size: 100 µl
Species: Human

Product manufactured in the USA

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Assay Background

Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of a representative isoprostane, 15-isoprostane F2t (also known as 8-epi-PGF2a, 8-iso-PGF2a or 8-isoprostane). Levels of 15-isoprostane F2t (15-IsoP F2t) in biological fluids have been shown to be useful for assessment of oxidant stress in vivo. 15-IsoP F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis.

The 15-Isoprostane F2t ELISA Assay kit can be used for the quantification of free 15-IsoP F2t in urine, plasma, serum, and tissue samples following Solid Phase Extraction (SPE) of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-IsoP F2t following hydrolysis of phospholipids.
NOTE: Alternatively, urine samples may be analyzed without SPE using a separately available kit, ISO34-K01 – Urinary Isoprostane ELISA, also available from Eagle Biosciences, Inc.

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