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Recombinant Humanalpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Reaction conditions will need to be optimized for each specific application.

Source

E. coli-derived human alpha-Synuclein protein

Accession #

P37840

Predicted Molecular Mass

14 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-486

Formulation	X mg/ml (XμM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 24 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE

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0.5 μg and 1 μg per lane of Recombinant Human alpha-Synuclein Low Endotoxin (Catalog # SP-486) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a single band at 19 kDa.

Reconstitution Calculator

Background: alpha-Synuclein

alpha -Synuclein is a member of a family of small, soluble proteins that also includebeta -, andgamma -Synuclein. alpha -Synuclein is predominantly expressed in neuronsof the central nervous system, specificallyin the presynaptic region of nerve terminals. This protein cycles between a free, partially unfolded form and a helical, membrane-bound form.alpha -Synuclein can aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms are major components of Lewy Bodies and have been linked to the pathogenesis of Parkinson"s disease dementia.alpha -Synuclein aggregates can also be found associated with amyloid plaques in Alzheimer"s disease. This product has an endotoxin level < 0.01 EU per 1μg of protein.

References

Breydo L., Wu J.W., Uversky V.N. (2012) Biochim Biophys Acta 1822: 261
Chen R.H. et al. (2013) J. Biol. Chem. 288:7438
Li X. et al. (2008) Acta. Biochim Biophys Sin (Shanghai) 40: 406
Surguchov A. (2008) Int Rev Cell Mol. Biol. 270: 225
Xia Q. et al. (2008) Front Biosci. 13:3850

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names

alpha-Synuclein; Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; SNCA; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha