Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF Summary
Ubiquilin 1 is a ubiquitously expressed member of the UBL-UBA family of Ubiquitin-binding proteins that is 589 amino acids (aa) in length and has a predicted molecular weight of 62.5 kDa (1). Human Ubiquilin 1 shares 88% aa sequence identity with the mouse and rat orthologs. It contains an N-terminal Ubiquitin-like (UBL) domain and a C-terminal Ubiquitin-associated (UBA) domain (2). The UBA domain is able to interact with mono- and poly-Ubiquitin chains, with a similar affinity for Lys48- and Lys63-linked chains (3,4). In contrast, the UBL domain interacts with Ubiquitin-interacting motif (UIM)-containing proteins, such as S5a/Angiocidin, a 19S Proteasome component (5). It is thought that Ubiquilin 1 may act as a molecular shuttle, bringing poly-ubiquitinated proteins to the 26S Proteasome by simultaneously binding poly-Ubiquitin via its UBA domain and the 19S Proteasome via its UBL domain (5). Ubiquilin 1 also binds two endoplasmic reticulum (ER)-localized proteins, Herp and Erasin, and may function in the ER-associated degradation pathway (6,7). It has been reported to co-precipitate with LC3/MAP1LC3A/Apg8p3, an autophagosomal marker, suggesting that it may play a role in autophagy (8,9). Single nucleotide polymorphisms in the UBQLN1 gene have been linked to Alzheimer"s disease (AD) (10). Furthermore, Ubiquilin 1 may regulate the processing of APP, which is associated with AD (11).
Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over the single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-ubiquitinated proteins, allowing for detection at relatively low abundance. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages.
Specifications
- 3 months from date of receipt, 2 to 8 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
Product Datasheets
Background: Ubiquilin 1
- Lee, D.Y. & E.J. Brown (2012) Biol. Chem. 393:441.
- Mah, A.L. et al. (2000) J. Cell Biol. 151:847.
- Raasi, S. et al. (2005) Nat. Struct. Mol. Biol. 12:708.
- Zhang, D. et al.(2008) J. Mol. Biol. 377:162.
- Ko, H.S. et al. (2004) FEBS Lett. 566:110.
- Kim, T.Y. et al. (2008) Biochem. Biophys. Res. Commun. 369:741.
- Lim, P.J. et al. (2009) J. Cell Biol. 187:201.
- N"Diaye, E.N. et al. (2009) EMBO Rep. 10:173.
- Rothenberg, C. et al. (2010) Hum. Mol. Genet. 19:3219.
- Bertram, L. et al. (2005) N. Engl. J. Med. 352:884.
- El Ayadi, A. et al. (2012) Proc. Natl. Acad. Sci. USA 109:13416.
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Citations for Recombinant Human Ubiquilin 1 Tandem UBA (TUBE2) Agarose, CF
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- DNA damage and transcription stress cause ATP-mediated redesign of metabolism and potentiation of anti-oxidant bufferingAuthors: C Milanese, CR Bombardier, S Sepe, S Barnhoorn, C Payán-Gomé, D Caruso, M Audano, S Pedretti, WP Vermeij, RMC Brandt, A Gyenis, MM Wamelink, AS de Wit, RC Janssens, R Leen, ABP van Kuilen, N Mitro, JHJ Hoeijmaker, PG MastroberaNat Commun, 2019;10(1):4887.2019
- Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomesAuthors: R Verma, KM Reichermei, AM Burroughs, RS Oania, JM Reitsma, L Aravind, RJ DeshaiesNature, 2018;0(0):.2018
- p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstratesAuthors: TV Nguyen, J Li, CJ Lu, JL Mamrosh, G Lu, BE Cathers, RJ DeshaiesProc. Natl. Acad. Sci. U.S.A, 2017;0(0):.2017
- Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11Authors: J Li, T Yakushi, F Parlati, AL Mackinnon, C Perez, Y Ma, KP Carter, S Colayco, G Magnuson, B Brown, K Nguyen, S Vasile, E Suyama, LH Smith, E Sergienko, AB Pinkerton, TD Chung, AE Palmer, I Pass, S Hess, SM Cohen, RJ DeshaiesNat. Chem. Biol, 2017;0(0):.2017
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Worked perfectly well for my project with C. elegans.
