Boston Biochem蚂蚁淘在线

Recombinant Human His6-USP19 is a ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP19 concentration of 10-100 nM.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP19 proteinMet1 - Arg1290, Contains a C-terminal 6-His tag

Accession #

O94966

Predicted Molecular Mass

143 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-576

Formulation	Xmg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP19

Ubiquitin specific processing protease 19 (USP19) is a 146 kDa member of the C19 peptidase family that contains two alpha crystallin domains and one MYND-type zinc finger. USP19 plays a role in the rescue of ER-associated protein degradation (ERAD) substrates such as cystic fibrosis transmembrane conductance regulator (CFTR)delta F508 and T-cell receptor-alpha (TCR alpha ) from proteasomal degradation. USP19 also interacts with the ubiquitin ligases cellular IAP 1 (cIAP1) and cIAP2. Knockdown of USP19 decreases levels of both cIAPs, whereas overexpression of USP19 results in a marked increase in cIAP levels. Although it effectively removes ubiquitin from cIAPs in vitro, USP19 may stabilize cIAP proteins in vivo mainly through deubiquitinase-independent mechanisms. Intriguingly, partial rescue of TCR alpha(but not CFTRdelta F508) in the ERAD pathway is also observed using a catalytically dead USP19, suggesting that USP19 exerts a non-catalytic function for select substrates in multiple biological pathways. This recombinant protein contains amino acids a C-terminal 6-His tag.

References

Hassink G.C., et al. (2009) EMBO Rep. 10: 755
Iphöfer, A. et al. (2012) ChemBioChem 13: 1416
Mei, Y. et al. (2011) J. Biol. Chem. 286: 35380

Long Name

Ubiquitin Specific Protease 19

Entrez Gene IDs

10869 (Human); 71472 (Mouse); 361190 (Rat)

Alternate Names

Deubiquitinating enzyme 19; EC 3.1.2.15; EC 3.4.19.12; KIAA0891; Ubiquitin Carboxyl-Terminal Hydrolase 19; ubiquitin specific peptidase 19; ubiquitin thioesterase 19; Ubiquitin Thiolesterase 19; Ubiquitin-specific-processing protease 19; USP19; Zinc finger MYND domain-containing protein 9; ZMYND9; ZMYND9ubiquitin specific protease 19