Recombinant Human AMSH/STAMBP (Sf 21-expressed) Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-548B
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl,10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: AMSH/STAMBP
AMSH (STAM binding protein) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). This enzyme functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. AMSH cleaves K63-linked but not K48-linked polyubiquitin chains, and the in vitro activity of AMSH is greatly increased in the presence of STAM protein. This recombinant protein is full-length.
- Agromayor, M. and Martin-Serrano, J. (2006) J. Biol. Chem 281:23083
- Kim, M.S., et al. (2006) Biochem. Biophys. Res Comm. 351:612
- McCullough, J., et al. (2006) Current Biology 16:160
- Sato, Y., et al. (2008) Nature. 455:358
Citations for Recombinant Human AMSH/STAMBP (Sf 21-expressed) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recoveryAuthors: P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT HuangElife, 2018;7(0):.Species: HumanSample Types: ProteinApplications: Bioassay
- Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activityAuthors: JS Bednash, N Weathingto, J Londino, M Rojas, DL Gulick, R Fort, S Han, AC McKelvey, BB Chen, RK MallampallNat Commun, 2017;8(0):15203.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
- Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11Authors: J Li, T Yakushi, F Parlati, AL Mackinnon, C Perez, Y Ma, KP Carter, S Colayco, G Magnuson, B Brown, K Nguyen, S Vasile, E Suyama, LH Smith, E Sergienko, AB Pinkerton, TD Chung, AE Palmer, I Pass, S Hess, SM Cohen, RJ DeshaiesNat. Chem. Biol, 2017;0(0):.Species: N/ASample Types: ProteinApplications: Bioassay
- Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activityAuthors: JS Bednash, N Weathingto, J Londino, M Rojas, DL Gulick, R Fort, S Han, AC McKelvey, BB Chen, RK MallampallNat Commun, 2017;8(0):15203.Species: HumanSample Types: ProteinApplications: Bioassay
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