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Recombinant Human GST-USP21 catalytic domain is a Ubiquitin deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP21 concentration of 10-200 nM.

Source

Trichoplusia ni, T. ni(baculovirus)-derived human USP21 proteinContains an N-terminal GST (glutathione S-transferase) tag, Ser196 - Leu565

Accession #

Q9UK80

Predicted Molecular Mass

68 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-622

Formulation	X mg/ml (XμM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 24 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP21

USP21 is a deubiquitinating enzyme (DUB) of the C19 peptidase family. Human USP21 has a predicted molecular weight of 63 kDa and is 96% identical to both mouse and rat orthologues. USP21 has been reported to deubiquitinate histone H2A, a specific tag for epigenetic transcriptional repression. The transcription factor FOXM1 is also reported to be a substrate for USP21 both in vitro and in vivo, and FOXM1 stabilization by USP21 contributes to cell cycle progression in basal-like breast cancer (BLBC). USP21 depletion sensitizes BLBC cell lines and mouse xenograft tumors to paclitaxel, a frontline therapy in BLBC treatment, suggesting USP21 inhibitors may be useful in cancer treatment. This recombinant protein contains an N-terminal GST tag and amino acid residues Ser 196 to Leu 565.

References

Arceci A., et al. (2019) Cell Rep. 26: 3076
Nakagawa T., et al. (2008) Genes Dev. 22: 37
Peng L., et al. (2016) Oncol. Lett. 12: 4531

Long Name

Ubiquitin Specific Protease 21

Entrez Gene IDs

27005 (Human); 30941 (Mouse); 688466 (Rat)

Alternate Names

BJ-HCC-26 tumor antigen; dual specificity phosphatase 21; dual specificity protein phosphatase 21; EC 3.1.3.16; EC 3.1.3.48; ESTM28; LMWDSP21; LMW-DSP21; Low molecular weight dual specificity phosphatase 21; MGC149878; USP21; USP23; W53272