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Recombinant Human His6-UBE2S is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2S concentration of 0.1-1 μM.

Source

E. coli-derived human UBE2S proteinContains an N-terminal 6-His tag

Accession #

NP_055316.2

Predicted Molecular Mass

36 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-690

Formulation	Xmg/ml (x μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UBE2S

Ubiquitin-conjugating Enzyme E2S (UBE2S), also known as E2-EPF, is a widely expressed protein with a predicted molecular weight of 26 kDa (1). Human UBE2S shares 92% and 93% amino acid sequence identity with mouse and rat UBE2S, respectively. UBE2S expression oscillates in a cell cycle-dependent fashion, and it influences cell cycle progression by mediating the Ubiquitin-dependent proteolysis of mitotic regulators (2-4). UBE2S interacts with APC/C, a Ubiquitin ligase (E3), to catalyze the elongation of Lys11-linked Ubiquitin chains on APC/C substrates that have been mono-ubiquitinated by UbcH10/UBE2C or UbcH5/UBE2D (3-7). UBE2S is overexpressed in multiple human cancers and targets the tumor suppressor pVHL for proteasomal degradation, which stabilizes HIF-1 alpha and promotes tumor progression (8).

References

Liu, Z. et al. (1992) J. Biol. Chem. 267:15829.
Tedesco, D. et al. (2007) Neoplasia 9:601.
Williamson, A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:18213.
Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
Garnett, M.J. et al. (2009) Nat. Cell Biol. 11:1363.
Wu, T. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1355.
Bremm, A. & D. Komander (2012) Methods Mol. Biol. 832:219.
Jung, C.R. et al. (2006) Nat. Med. 12:809.

Additional Resources

Chen M.F., et al. (2009) J. Mol. Med. 87:307-320
Jung C.-R., et al. (2006) Nature Med. 12:809-816
Liu Z., et al. (1992) J. Biol. Chem. 267:15829-15835
Lim J.H., et al. (2008) J. Cell. Biochem. 105:1117-1127
Ohh M. (2006) Cancer Cell 10:95-97

Long Name

Ubiquitin-conjugating Enzyme E2S

Entrez Gene IDs

27338 (Human); 292588 (Rat)

Alternate Names

E2-24K; E2-EPF; E2-EPF5; E2-EPF52; E2-EPFEC 6.3.2.19; E2EPFEPF5; EPF5; UBE2S; Ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S

Related Research Areas

Adhesion Molecules in Angiogenesis
Cancer Biomarkers
E2 Ubiquitin/Ubl Conjugating Enzymes
Immunoglobulin Superfamily CAMs
Search Ubiquitin-related Enzymes

Citations for Recombinant Human His6UBE2S Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

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Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortaseAuthors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K LangNat. Chem. Biol., 2019;15(3):276-284.Species: HumanSample Types: Recombinant ProteinApplications: Enzyme Assay
Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkageClin Cancer Res, 2016;0(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination