Recombinant Human UbcH8/UBE2L6 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-644
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UbcH8
Ubiquitin-conjugating Enzyme H8 (UbcH8), also known as Ubiquitin-conjugating Enzyme E2L 6 (UBE2L6), has a predicted molecular weight of 18 kDa. Human UbcH8 shares 76% and 74% amino acid sequence identity with mouse and rat UBE2L6, respectively. UbcH8 is capable of transferring either Ubiquitin or the Ubiquitin-like protein ISG15 Ubiquitin-like Modifier (ISG15) to target proteins (1,2). ISG15 conjugation is mediated by UbcH8 following IFN-alpha or IFN-beta stimulation (3,4). UbcH8 interacts with a variety of Ubiquitin ligases (E3s) to transfer Ubiquitin to target proteins such as the cell cycle regulator, p21/CIP1/CDKN1A (5). UbcH8 also regulates actin cytoskeleton dynamics, and dysregulated UbcH8 promotes migration and invasion of human breast cancer cells (6). In addition, UbcH8 interacts with the E3, Parkin, and is thought to play a role in the pathogenesis of Parkinson’s disease (1,7).
- Kumar, S. et al. (1997) J. Biol. Chem. 272:13548.
- Durfee, L.A. & J.M. Huibregste (2012) Methods Mol. Biol. 832:141.
- Zhao, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:7578.
- Kim, K. et al. (2004) Mol. Cell. Biol. 24:9592.
- Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
- Desai, S.D. et al. (2012) Exp. Biol. Med. 237:38.
- Zhang, Y. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13354.
FAQs
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