Recombinant Human UbcH7/UBE2L3 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-640
| Formulation | Xmg/ml (X μM) in 50 mMHEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v),1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UbcH7/UBE2L3
Ubiquitin-conjugating Enzyme H7 (UbcH7), also known as Ubiquitin-conjugating Enzyme E2L 3 (UBE2L3), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). It has a predicted molecular weight of approximately 18 kDa. The human UbcH7 protein shares 100% amino acid (aa) sequence identity with the mouse and rat orthologs. UbcH7 has an E2 catalytic core domain that contains an active site cysteine residue and comprises 152 of its 154 aa residues. UbcH7 is catalytically active with HECT and RBR domain-containing families of Ubiquitin ligases (E3s) (2,3). UbcH7 localizes to both the nucleus and cytoplasm in human cells. In mice, its ortholog is expressed in many tissues including brain, muscle, heart, lung, lymph node, spleen, thymus, and testis (4,5). UbcH7 depletion results in an extended S phase and a reduced rate of proliferation, suggesting that it may play a role in the cell cycle (6). In humans, single nucleotide polymorphisms in UbcH7 are associated with systemic lupus erythematosus and Crohn"s disease, suggesting that UbcH7 is important for proper immune system function (7,8).
- Nuber, U. et al. (1996) J. Biol. Chem. 271:2795.
- Huang, L. et al. (1999) Science 286:1321.
- Wenzel, D.M. et al. (2011) Nature 474:105.
- Garside, H. et al. (2006) J. Endocrinol. 190:621.
- Harbers, K. et al. (1996) Proc. Natl. Acad. Sci. USA 93:12412.
- Whitcomb, E.A. et al. (2009) Mol. Biol. Cell 20:1.
- Wang, S. et al. (2012) Genes Immun. 13:380.
- Fransen, K. et al. (2010) Hum. Mol. Genet. 19:3482.
Citations for Recombinant Human UbcH7/UBE2L3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signalingAuthors: Q Yin, T Han, B Fang, G Zhang, C Zhang, ER Roberts, V Izumi, M Zheng, S Jiang, X Yin, M Kim, J Cai, EB Haura, JM Koomen, KSM Smalley, L WanNat Commun, 2019;10(1):1870.Species: HumanSample Types: Recombinant ProteinApplications: Ubiquitination
- The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase FunctionAuthors: S He, Y Cao, P Xie, G Dong, L ZhangSci Rep, 2017;7(0):41364.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disabilityAuthors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van LoonSci Rep, 2017;7(1):15050.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesisAuthors: X Zhang, B Li, AH Rezaeian, X Xu, PC Chou, G Jin, F Han, BS Pan, CY Wang, J Long, A Zhang, CY Huang, FJ Tsai, CH Tsai, C Logothetis, HK LinNat Commun, 2017;8(0):14799.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
- Mechanism of Ubiquitin Chain Synthesis Employed by a HECT Ubiquitin LigaseAuthors: ME French, JL Klosowiak, A Aslanian, SI Reed, JR Yates, T HunterJ. Biol. Chem., 2017;0(0):.Applications: Bioassay
- Monoubiquitination Inhibits the Actin Bundling Activity of FascinAuthors: Shengyu YangJ. Biol. Chem., 2016;0(0):.Applications: Bioassay
- Smurf2 negatively modulates RIG-I-dependent antiviral response by targetingVISA/MAVS for ubiquitination and degradation.Authors: Pan Y, Li R, Meng J, Mao H, Zhang Y, Zhang JJ Immunol, 2014;192(10):4758-64.Species: HumanSample Types: Whole Cells
- Ubiquitination increases parkin activity to promote autophagic alpha-synucleinclearance.Authors: Lonskaya I, Desforges N, Hebron M, Moussa CPLoS ONE, 2013;8(12):e83914.Species: N/ASample Types: Recombinant ProteinApplications: Enzyme Assay
- Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6).Authors: Hebron, Michaeli, Lonskaya, Irina, Sharpe, Kaydee, Weerasinghe, Puwakdan, Algarzae, Norah K, Shekoyan, Ashot R, Moussa, CharbelJ Biol Chem, 2013;288(6):4103-15.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
- Characterization of ML-IAP protein stability and physiological role in vivo.Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, DomagojBiochem J, 2012;447(3):427-36.Species: HumanSample Types: ProteinApplications: Bioassay
- ERalpha phosphorylation at Y537 by Src triggers E6-AP-ERalpha binding, ERalphaubiquitylation, promoter occupancy, and target gene expression.Authors: Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland JMol Endocrinol, 2012;26(9):1567-77.Species: HumanSample Types: ProteinApplications: Bioassay
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From the attached image, the first lane is reaction with this product, while the second lane is without it. We can see the difference between the ubiquitination smears is very significant.
