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Recombinant Human UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5c/UBE2D3 concentration of 0.1-1 μM.

Source

E. coli-derived human UbcH5c/UBE2D3 protein

Accession #

P61077

Predicted Molecular Mass

17 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-627

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UbcH5c/UBE2D3

Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).

References

Page, R.C. et al. (2012) Biochemistry 51:4175.
Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
Shembade, N. et al. (2010) Science 327:1135.
Xia, Z.P. et al. (2009) Nature 461:114.
Zhang, S. et al. (2008) Cell Cycle 7:3399.
Bentley, M.L. et al. (2011) EMBO J. 30:3285.
Polanowska, J. et al. (2006) EMBO J. 25:2178.

Entrez Gene IDs

7323 (Human); 66105 (Mouse); 81920 (Rat)

Alternate Names

E2(17)KB 3; E2(17)KB3; EC 6.3.2.19; MGC43926; MGC5416; UBC4/5; UBC5C; UbcH5c; UBE2D3; Ubiquitin carrier protein D3; ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3

Related Research Areas

E2 Ubiquitin/Ubl Conjugating Enzymes
Search Ubiquitin-related Enzymes

Citations for Recombinant Human UbcH5c/UBE2D3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

33Citations: Showing 1 - 10Filter your results:

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Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural PatterningAuthors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B MaoCell Rep, 2019;28(2):541-553.e4.Applications: Bioassay
The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling PathwaysAuthors: CM Xie, M Tan, XT Lin, D Wu, Y Jiang, Y Tan, H Li, Y Ma, X Xiong, Y SunCell Rep, 2019;26(11):3037-3050.e4.Applications: Bioassay
Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome developmentAuthors: P Wang, F Yan, Z Li, Y Yu, SE Parnell, Y XiongJ. Clin. Invest., 2019;130(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent mannerAuthors: B Liu, J Yi, X Yang, L Liu, X Lou, Z Zhang, H Qi, Z Wang, J Zou, WG Zhu, W Gu, J LuoOncogene, 2018;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay
The MtDMI2-MtPUB2 negative feedback loop plays a role in nodulation homeostasisAuthors: J Liu, J Deng, F Zhu, Y Li, Z Lu, P Qin, T Wang, J DongPlant Physiol., 2018;0(0):.Species: Plant - Medicago truncatulaSample Types: Recombinant ProteinApplications: Bioassay
PCGF5 is required for neural differentiation of embryonic stem cellsAuthors: M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H YaoNat Commun, 2018;9(1):1463.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndromeAuthors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES FischerElife, 2018;7(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination
The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase FunctionAuthors: S He, Y Cao, P Xie, G Dong, L ZhangSci Rep, 2017;7(0):41364.
H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesisAuthors: X Zhang, B Li, AH Rezaeian, X Xu, PC Chou, G Jin, F Han, BS Pan, CY Wang, J Long, A Zhang, CY Huang, FJ Tsai, CH Tsai, C Logothetis, HK LinNat Commun, 2017;8(0):14799.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
Ubiquitination and dynactin regulate TMEPAI lysosomal traffickingAuthors: S Luo, L Jing, T Zhao, Y Li, Z Liu, A DiaoSci Rep, 2017;7(0):42668.Species: HumanSample Types: Cell LysatesApplications: Bioassay

C-terminus of HSC70-Interacting Protein (CHIP) Inhibits Adipocyte Differentiation via Ubiquitin- and Proteasome-Mediated Degradation of PPAR?Authors: JH Kim, S Shin, J Seo, EW Lee, M Jeong, MS Lee, HJ Han, J SongSci Rep, 2017;7(0):40023.Species: HumanSample Types: Cell LysatesApplications: Ubiquitination

Ubiquitylation of the acetyltransferase MOF in Drosophila melanogasterAuthors: S Schunter, R Villa, V Flynn, JB Heidelberg, AK Classen, P Beli, PB BeckerPLoS ONE, 2017;12(5):e0177408.Species: Worm - Spodoptera frugiperda (Fall Army Worm)Sample Types: ProteinApplications: Bioassay

Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disabilityAuthors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van LoonSci Rep, 2017;7(1):15050.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repairAuthors: JR Brickner, JM Soll, PM Lombardi, CB Vågbø, MC Mudge, C Oyeniran, R Rabe, J Jackson, ME Sullender, E Blazosky, AK Byrum, Y Zhao, MA Corbett, J Gécz, M Field, A Vindigni, G Slupphaug, C Wolberger, N MosammaparNature, 2017;551(7680):389-393.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometryAuthors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K HusnjakNat. Methods, 2017;0(0):.Species: N/ASample Types: ProteinApplications: Bioassay

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancerAuthors: SY Kim, HJ Kim, HJ Kim, DH Kim, CH Kim, JH Han, HK Byeon, K LeeAutophagy, 2017;0(0):1-89.Species: HumanSample Types: Cell LysatesApplications: Bioassay

The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosisAuthors: Y Xu, Z Zhang, J Li, J Tong, B Cao, P Taylor, X Tang, D Wu, MF Moran, Y Zeng, X MaoJ Hematol Oncol, 2017;10(1):132.Species: HumanSample Types: ProteinApplications: Bioassay

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during SpermiogenesisAuthors: LT Gou, JY Kang, P Dai, X Wang, F Li, S Zhao, M Zhang, MM Hua, Y Lu, Y Zhu, Z Li, H Chen, LG Wu, D Li, XD Fu, J Li, HJ Shi, MF LiuCell, 2017;0(0):.Applications: Bioassay

In vitro characterization of six STUB1 variants in spinocerebellar ataxia 16 reveals altered structural properties for the encoded CHIP proteinsAuthors: Y Pakdaman, M Sanchez-Gu, R Kleppe, S Erdal, HJ Bustad, L Bjørkhaug, K Haugarvoll, C Tzoulis, K Heimdal, PM Knappskog, S Johansson, I AukrustBiosci. Rep., 2017;37(2):.Species: HumanSample Types: ProteinApplications: Bioassay

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosisAuthors: S Wang, J Juan, Z Zhang, Y Du, Y Xu, J Tong, B Cao, MF Moran, Y Zeng, X MaoCell Death Dis, 2017;8(9):e3058.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformationAuthors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M CarboneNature, 2017;546(7659):549-553.Species: N/ASample Types: ProteinApplications: Bioassay

RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexesElife, 2016;5(0):.Applications: Bioassay

Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stressSci Rep, 2016;6(0):36171.Species: HumanSample Types: ProteinApplications: Bioassay

GpDSR7, a Novel E3 Ubiquitin Ligase Gene in Grimmia pilifera Is Involved in Tolerance to Drought Stress in ArabidopsisPLoS ONE, 2016;11(5):e0155455.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

TRAF6 Restricts p53 Mitochondrial Translocation, Apoptosis, and Tumor SuppressionAuthors: Hui-Kuan LinMol. Cell, 2016;64(4):803-814.Applications: Bioassay

Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang SNat Commun, 2015;6(0):6156.Species: HumanSample Types: ProteinApplications: Bioassay

Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cellsin vivo.Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai MNat Commun, 2015;6(0):6353.Species: N/ASample Types: ProteinApplications: Bioassay

Suppression of PPARgamma through MKRN1-mediated ubiquitination and degradationprevents adipocyte differentiation.Authors: Kim J, Park K, Lee E, Jang W, Seo J, Shin S, Hwang K, Song JCell Death Differ, 2014;21(4):594-603.Species: HumanSample Types: Recombinant ProteinApplications: Enzyme Assay

Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception.Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven YElife, 2014;3(0):.Species: N/ASample Types: ProteinApplications: Bioassay

Cytosolic quality control of mislocalized proteins requires RNF126 recruitment toBag6.Authors: Rodrigo-Brenni M, Gutierrez E, Hegde RMol Cell, 2014;55(2):227-37.Applications: Bioassay

ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou YMol Cell, 2013;49(6):1108-20.

Characterization of ML-IAP protein stability and physiological role in vivo.Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, DomagojBiochem J, 2012;447(3):427-36.Species: HumanSample Types: ProteinApplications: Bioassay

A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay LJ Biol Chem, 2012;287(52):43410-6.Species: HumanSample Types: Recombinant ProteinApplications: Ubiquitination