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Reaction conditions will need to be optimized for each specific application. We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555),or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP30 proteinContains a C-terminal 6-His tag, Thr57 - Glu517

Accession #

Q70CQ3

Predicted Molecular Mass

54 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-582

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.2, 0.4 M NaCl, 10% Glycerol (v/v), 2 mM DTT
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP30

Ubiquitin carboxyl-terminal hydrolase 30 (USP30) is a member of the C19 family of peptidases and is tethered to the outer mitochondrial surface via a trans-membrane domain. USP30 activity is antagonistic to the pro-mitophagy signal generated by the activities of E3 Ubiquitin Ligase Parkin (PARK2) and kinase PINK1, whichare stimulated by mitochondrial damage. In vivo, USP30 cleaves Ubiquitin from mitochondrial surface proteins such as MIRO1, TOMM20, MFN1, and MFN2. The enzyme displays a strong preference for K6- and K11-linked Polyubiquitin chains in vitro, but has substantially lower activity on Polyubiquitin chains that have been phosphorylated with PINK1. This recombinant protein contains amino acids 57-517 of the full-length protein, and a C-terminal 6-His tag.

References

Bingol B. et al. (2014) Nature 510: 370
Cunningham C.N. et al. (2015) Nat Cell Biol. 17: 160
Thobois S. (2015) Mov. Disord. 30: 340
Wang, Y. et al. (2015) Autophagy 11: 595
Wauer T., et al. (2015) EMBO J. 34: 307
Yue, W. et al. (2014) Cell Res. 24: 482

Long Name

Ubiquitin Specific Protease 30

Entrez Gene IDs

84749 (Human); 100756 (Mouse); 304579 (Rat)

Alternate Names

Deubiquitinating Enzyme 30; EC 3.1.2.15; EC 3.4.19.12; FLJ40511; MGC10702; ubiquitin carboxyl-terminal hydrolase 30; ubiquitin specific peptidase 30; ubiquitin specific protease 30; Ubiquitin Thioesterase 30; Ubiquitin thiolesterase 30; ubiquitin-specific protease 30; Ubiquitin-specific-processing protease 30; Ub-specific protease 30; USP30