Boston Biochem蚂蚁淘在线

Ubiquitin chains vary in length, linkage and function. K11/K48-linked, "branched" Tri-ubiquitin chains are ideal as a Western Blot control as described in Yau et al. in "Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control."

Source

E. coli-derived human Tri-Ubiquitin protein

Accession #

P0CG47

Predicted Molecular Mass

26 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UC-1148

Formulation	0.5 mg/ml (20μM) in 10 mM HEPES pH 7.5
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 24 months from date of receipt, -70 °C as supplied. 12 months, -20 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Tri-Ubiquitin

Linkage-specific polyubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide of isopeptide linkage between adjacent ubiquitin molecules. Polyubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBA"s), Ubiquitin-Interacting Motifs (UIM"s), ZnF"s, and/or other ubiquitin-sensing elements.

K11/K48-branched polyubiquitin chains facilitate rapid substrate delivery to the proteasome, which prevents accumulation of aggregation-prone nascent proteins. Furthermore, enzymes and effectors of K11/K48-linked chains are encoded by essential genes that are often mutatedin neurodegenerative diseases, providing proof that heterotypic ubiquitin chains play important roles in cellular signaling pathways.

The correctness of linkage and purity of each production lot of this product is assessed using the Absolute Quantitation of Ubiquitin Method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information of the composition of polyubiquitin samples. The product is also tested in a Western Blot application described by Yau et al.

References