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Recombinant Human His6-UbcH10/UBE2C is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH10/UBE2C concentration of 0.1-1 μM.

Source

E. coli-derived human UbcH10/UBE2C proteinContains an N-terminal Met and 7-His tag

Accession #

O00762

Predicted Molecular Mass

21 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-650

Formulation	Xmg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UbcH10/UBE2C

Ubiquitin-conjugating Enzyme H10 (UbcH10), also known as Ubiquitin-conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes and has a predicted molecular weight of 20 kDa. Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C. UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression (1,2). UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11-linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin ligase (E3) (3). The catalytic activity of UbcH10/UBE2C is regulated by a conserved N-terminal extension, which mediates E2-E3 interaction (4). UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression (5-8).

References

Ye, Y. & M. Rape (2009) Nat. Rev. Mol. Cell Biol. 10:755.
Lin, Y. et al. (2002) J. Biol. Chem. 277:21913.
Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
Summers, M.K. et al. (2008) Mol. Cell 31:544.
Okamoto, Y. et al. (2003) Cancer Res. 63:4167.
van Ree, J.H. et al. (2010) J. Cell Biol. 188:83.
Jiang, L. et al. (2008) Brain Res. 1201:161.
Hao, Z. et al. (2012) Tumor Biol. 33:723.

Entrez Gene IDs

11065 (Human); 68612 (Mouse); 296368 (Rat)

Alternate Names

cyclin-selective ubiquitin carrier protein; dJ447F3.2; EC 6.3.2.19; UbcH10; UBCH10mitotic-specific ubiquitin-conjugating enzyme; UBE2C; Ubiquitin carrier protein C; ubiquitin carrier protein E2-C; ubiquitin-conjugating enzyme E2 C; ubiquitin-conjugating enzyme E2C; Ubiquitin-protein ligase C