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蚂蚁淘在线 / 品牌中心 / Boston Biochem / Boston Biochem/Recombinant Human His10-USP47 Protein, CF/E-626-050

Boston Biochem/Recombinant Human His10-USP47 Protein, CF/E-626-050

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￥5900.00
货号：E-626-050
浏览量：72
品牌：Boston Biochem
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human His10-USP47 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human USP47 is a Ubiquitin deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial concentration of 10-100 nM USP47 when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine (U-555) as a substrate.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin proteinContains an N-terminal 10-His tag
Predicted Molecular Mass
160 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-626

FormulationX mg/ml (XμM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP
ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin carboxyl-terminal hydrolase 47 (USP47) is a C19 type peptidase with a predicted molecular weight of 157 kDa. The human protein shares 95% and 96% amino acid sequence identity with its mouse and rat orthologs, respectively. USP47 has been reported to play roles in inflammasome activation and colon cancer progression. This recombinant protein contains an n-terminal 10-His tag.

References
  1. Palazon-Riquelme, P. et al. (2018) EMBO Rep. doi: 10.15252/embr.201744766
  2. Yu, L. et al. (2019) Cancer Letters doi: 10.1016/j.canlet.2019.01.039
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

FAQs

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Boston Biochem波士顿生物化学已与IQ Proteomics™合作，为分析您的泛素化蛋白提供定制服务。使用哈佛医学院Gygi实验室开发的泛素绝对定量（AQUA）技术，我们可以对样品中存在的不同泛素连接进行定量表征。