More Information Product Details Synonyms Product Type Properties Source/Host Sequence Crossreactivity Label/Conjugates Formulation Other Product Data Declaration Shipping and Handling Shipping Short Term Storage Long Term Storage Handling Advice Use/Stability Documents MSDS Product Specification Sheet Datasheet

Ubiquitin-conjugating Enzyme E2; E2-25K; Hip2; HYPG; LIG; UBE2K; UBE2K-25K; Huntingtin-interacting Protein 2

Protein

E. coli

Human UbcH1 (Accession Nr. P61086) conjugated to biotin.

Human

Biotin

Liquid.

Use: Can be used as a positive control for sumoylation assays. It is modified by SUMO-1, SUMO-2 and SUMO-3 proteins and can be detected with avidin-linked reagents. Typical concentration to use in SUMO conjugation experiments in vitro is 1-5μM depending on conditions.

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -80°C.

No

Download PDF

Ubiquitin-conjugating Enzyme E2K (UBE2K), also known as UbcH1, E2-25K, HIP2 and LIG, is a 200 amino acid (aa) protein with a predicted molecular weight of 25 kDa. Human and mouse UbcH1 share 100% aa sequence identity. Similar to other ubiquitin-conjugating (E2) enzymes, UbcH1 contains a conserved ubiquitin-conjugating domain but is unique in that it also has a C-terminal, non-covalent ubiquitin binding surface termed the ubiquitin-associated domain between aa residues 160 and 200. UbcH1 mediates the elongation of Lys48-linked poly-ubiquitin chains. UbcH1 catalytic activity can be modulated by the post-translational addition of SUMO at Lys14. Substrates include the Huntingtin protein and the tumor suppressor RB1. UbcH1 is widely expressed, with highest levels found in the brain cortex and striatum, and dysregulated UbcH1 is implicated in polyglutamine diseases and Alzheimers disease.

>>> 更多资讯详情请访问蚂蚁淘商城