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Ubiquitin-conjugating Enzyme H5c; UBC5C; Ubiquitin-protein Ligase D3; UBE2D3

Protein

E. coli

Human UbcH5c (Accession Nr. P61077) fused to a N-terminal GST-tag.

Human

Liquid. In 50mM HEPES pH 7.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.

Use: Member of the ubiquitin­conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin­activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-­1μM.

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -80°C.

No

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Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins. For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the NF-κB inhibitor, IκB-α, and is implicated in NF-κB-dependent inflammation. UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication and DNA damage responses.

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