Recombinant Human His6-UBE2N/Ubc13 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-660
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: UBE2N/Ubc13
Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2N/Ubc13 has a predicted molecular weight of 17 kDa. The human enzyme shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively. UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). UBE2N/Ubc13 localizes to both the nucleus and cytoplasm (2). UBE2N/Ubc13 forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants. The UBE2N (Ubc13)/Uev1A Complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor kappaB (NF-kappa B) activation (3,4). In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response (3,5-7). Pathologically, UBE2N (Ubc13)/Uev1a Complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells (8). This protein has an N-terminal His6-tag.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Laine, A. et al. (2006) Mol. Cell. Biol. 26:8901.
- Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
- Pertel, T. et al. (2011) Nature 472:361.
- Zhao, G.Y. et al. (2007) Mol. Cell 25:663.
- Stewart, G.S. et al. (2009) Cell 136:420.
- Campbell, S.J. et al. (2012) J. Biol. Chem. 287:23900.
- Pulvino, M. et al. (2012) Blood 120:1668.
Citation for Recombinant Human His6-UBE2N/Ubc13 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2NAuthors: N Gan, ES Nakayasu, PJ Hollenbeck, ZQ LuoNat Microbiol, 2019;4(1):134-143.Species: HumanSample Types: ProteinApplications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsRecombinant Enzymes
Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
Recombinant Proteins
Recombinant Human SUMO2 Protein, CF
Recombinant Human Ubiquitin Protein, CF
Ubiquitin E3 Ligase Kits
Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate
Reviews for Recombinant Human His6-UBE2N/Ubc13 Protein, CF
There are currently no reviews for this product. Be the first toreview Recombinant Human His6-UBE2N/Ubc13 Protein, CF and earn rewards!
Have you used Recombinant Human His6-UBE2N/Ubc13 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
