Recombinant Human His6-USP13 Protein, CF Summary
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-588
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: USP13
Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages. Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.
- Xiang, S. et al. (2015) Oncol Rep. 33: 1342
- Zhang, J. et al. (2013) Nat Cell Biol. 15: 1486
- Zhang, Y-H. et al. (2011) PLoS One 6: e29362
- Zhao, X. et al. (2011) Nat Commun. 2: 414. doi: 10.1038/ncomms1421
Citation for Recombinant Human His6-USP13 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitorsAuthors: S Zhang, M Zhang, Y Jing, X Yin, P Ma, Z Zhang, X Wang, W Di, G ZhuangNat Commun, 2018;9(1):215.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsAffinity Matrices
Recombinant Human His6-RAP80 UIM Domains Protein, CF
Recombinant Enzymes
Recombinant Human His6-USP10 Protein, CF
Recombinant Human PTEN Protein, CF
Recombinant Proteins
Recombinant Human FAT10 Rhodamine Protein, CF
Recombinant Human Mcl-1 Western Blot Standard Protein
Recombinant Human Ubiquitin Protein, CF
Recombinant Human ISG15 AMC Protein, CF
Reviews for Recombinant Human His6-USP13 Protein, CF
There are currently no reviews for this product. Be the first toreview Recombinant Human His6-USP13 Protein, CF and earn rewards!
Have you used Recombinant Human His6-USP13 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
