Recombinant Human His6-NEDP1/SENP8 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-800
| Formulation | Xmg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: NEDP1/SENP8
NEDD8-specific Protease 1 (NEDP1), also known as SUMO/Sentrin Specific Peptidase Family Member 8 (SENP8), DEN1, PRSC2, and FKSG8, is a cytoplasmic cysteine protease that belongs to the ULP/peptidase C48 family of deubiquitinating enzymes (1). It is widely expressed with the highest levels being observed in kidney and pancreas (2). NEDP1/SENP8 is 212 amino acids (aa) in length with a predicted molecular weight of 24.1 kDa (1). It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Human NEDP1/SENP8 shares 92% aa sequence identity with the mouse and rat orthologs. NEDP1/SENP8 is involved in NEDD8-associated ubiquitination (1,2,3-5). NEDD8 is a small Ubiquitin-like protein that covalently binds to target molecules, including Cullin 1 and p53 (6-8). It is a critical regulator of cell proliferation and development (3). NEDP1/SENP8 influences the NEDDylation of proteins by converting pro-NEDD8 into its mature form and deconjugating NEDD8 from its bound substrates (2,3-5,9).
- Gan-Erdene, T. et al. (2003) J. Biol. Chem. 278:28892.
- Mendoza, H.M. et al. (2003) J. Biol. Chem. 278:25637.
- Wu, K. et al. (2003) J. Biol. Chem. 278:28882.
- Shen, L. et al. (2005) EMBO J. 24:1341.
- Shin, Y.C. et al. (2011) PLoS One 6:e27742.
- Pan, Z.Q. et al. (2004) Oncogene 23:1985.
- Xirodimas, D.P. et al. (2004) Cell 118:83.
- Xirodimas, D.P. et al. (2008) EMBO Rep. 9:280.
- Ehrentraut, S.F. et al. (2012) J. Immunol. [Epub ahead of print].
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