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Recombinant Human His6-ZRANB1/Trabid is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ZRANB1/Trabid concentration of 0.1-1 μM.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ZRANB1/Trabid proteinContains a C-terminal 6-His tag

Accession #

Q9UGI0

Predicted Molecular Mass

82 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-560

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: ZRANB1/Trabid

Zinc Finger, RAN-binding Domain Containing 1 (ZRANB1), also known as TRAF-binding Domain-containing Protein (Trabid), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 81 kDa (1). The human protein shares 99% amino acid sequence identity with its mouse ortholog. ZRANB1 preferentially cleaves K29-, K33-, and K63-linked poly-Ubiquitin chains (2). It has been shown to play a role in the regulation of Wnt signaling via deubiquitination of APC (3,4). This recombinant protein contains a C-terminal 6-His tag.

References

Evans, P.C. et al. (2001) Biochem. J. 357:617.
Licchesi, J.D. et al. (2011) Nat. Struct. Mol. Biol. 19:62.
Tran, H. et al. (2008) Genes Dev. 22:528.
Shi, T. et al. (2012) BMC Chem. Biol. 12:4.

Long Name

Zinc Finger, RAN-binding Domain Containing 1

Entrez Gene IDs

54764 (Human); 360216 (Mouse)

Alternate Names

HRPE773; jacalin-like lectin domain containing 2; JCLN2; pancreatic adenocarcinoma upregulated factor; PAUF; PRO1567; Trabid; ZRANB1; zymogen granule protein 16 homolog B (rat); zymogen granule protein 16 homolog B