Recombinant Human His6-UBE2Q2 Isoform 1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-745
| Formulation | X mg/ml (X μM) in 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2Q2
Ubiquitin-conjugating Enzyme E2Q Family Member 2 (UBE2Q2) is a 375 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 43 kDa. Human UBE2Q2 shares 95% aa sequence identity with its mouse ortholog. UBE2Q2 contains an RWD domain, a coiled-coil domain, and an E2 catalytic core domain (1). The E2 cataylic core domain contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2,3). UBE2Q2 expression has been observed in both the cytoplasm and the nucleus (1,4). Expression of dominant-negative UBE2Q2 can cause a prolonged prophase arrest and UBE2Q2 overexpression inhibits cell proliferation, suggesting that UBE2Q2 may be involved in normal cell cycle progression (3,4). Elevated expression of UBE2Q2 has been reported in hypopharyngeal cancer, breast cancer, and acute lymphoblastic leukemia, suggesting that this enzyme may function as an oncogene (1,4-6). This protein contains an N-terminal 6-His tag.
- Seghatoleslam, A. et al. (2006) Biochem. Biophys. Res. Commun. 339:422.
- Melner, M.H. et al. (2006) Biol. Reprod. 75:395.
- Banerjee, S. et al. (2007) Oncogene 26:6509.
- Maeda, H. et al. (2009) Mol. Cancer Res. 7:1553.
- Nikseresht, M. et al. (2010) Cancer Genet. Cytogenet. 197:101.
- Seghatoleslam, A. et al. (2012) Arch. Iran. Med. 15:352.
FAQs
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