Recombinant Human His6-Ufc1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-675
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ufc1
Ubiquitin-fold Modifier Conjugating Enzyme 1 (Ufc1) has been identified as an E2-like conjugating enzyme for Ubiquitin Fold Modifier 1 (Ufm1) (1). Ufc1 is a 116 amino acid (aa) protein with a predicted molecular weight of 19 kDa. Human Ufc1 exhibits 97% and 98% aa identity to its mouse and rat orthologs, respectively. The process of attaching Ufm1 to target proteins is termed ufmylation. It is thought to occur in a manner analogous to the ubiquitin system, including stepwise enzymatic reactions involving Ubiquitin-activating (E1)-, Ubiquiting-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. In addition to Ufc1, other members of the Ufm1-conjugation system include the E1-like enzyme Uba5, E3-like enzyme Ufl1, and deconjugating enzymes UfSP1 and UfSP2 (1-3). This protein contains an N-terminal His6 tag.
- Komatsu, J. et al. (2004) EMBO J. 23:1977.
- Tatsumi, K. et al. (2010) 285:5417.
- Kang, S.H. et al. (2007) J. Biol. Chem. 282:5256.
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