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Recombinant Human USP16 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. USP16 does not efficiently utilize Ubiquitin-AMC (U-550), Ubiquitin-Rhodamine 110 (U-555), norunanchored poly-Ubiquitin chains as substrates in vitro. USP16 cleaves Ubiquitin from recombinant, designer nucleosomes.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP16 protein

Accession #

Q9Y5T5

Predicted Molecular Mass

94 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-612

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP16

USP16 is a deubiquitinating enzyme (DUB) of the C19 peptidase family, USP16 subfamily. Human USP16 has a predicted molecular weight of 94 kDa and is 83% identical to both mouse and rat orthologues. USP16 plays significant roles in cell-cycle progression and Hox gene expression, and USP16 knockdown in HeLa cells results in defects in the mitotic phase of the cell cycle. Studies reveal that histone H2A deubiquitination by USP16 is a prerequisite for phosphorylation of serine 10 of histone H3 and chromosome segregation during mitosis. USP16 specifically deubiquitinates histone H2A, but not H2B, both in vitro and in vivo. PLK1 serine/threonine kinase is another reported target of USP16. Finally, a chromosomal inversion involving USP16 and RUNX1/AML1 is a causative event in the development of chronic myelomonocytic leukemia (CMML). This recombinant protein contains an N-terminal 6-His tag.

References

Cai S.Y., Babbitt R.W., Marchesi V.T.(1999) Proc. Natl. Acad. Sci. 96: 2828
Joo H.Y., et al. (2007) Nature 499: 1068
Gelsi-Boyer V., et al. (2008) BMC Cancer 8: 299
Zhuo X., et al. (2015) J. Cell Biol. 210: 727

Long Name

Ubiquitin Specific Protease 16

Entrez Gene IDs

10600 (Human); 74112 (Mouse); 288306 (Rat)

Alternate Names

dual specificity phosphatase 16; EC 3.1.3.16; EC 3.1.3.48; FLJ36298; FLJ40991; KIAA1700MGC129701; MAP kinase phosphatase 7; MAPK phosphatase-7; Mitogen-activated protein kinase phosphatase 7; MKP7dual specificity protein phosphatase 16; MKP-7MGC129702; UBP-M; USP16