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Recombinant human USP24 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP24 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine 110 substrates.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP24 protein

Predicted Molecular Mass

296 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-616

Formulation	X mg/ml (X μM) is 50 mM HEPES, pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT, 1 mM EDTA
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 24 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP24

USP24 is a deubiquitinating enzyme (DUB) of the C19 peptidase family. Human USP24 has a predicted molecular weight of 294 kDa and is 98% identical to the mouse orthologue. USP24 reportedly deubiquitinates a wide variety of proteins such as p300, Bax, E2F4, securin, p53 and Mcl-1. In one study, decreased USP24 activity correlated with decreased cell apoptosis and increased cell cycle progression seen in samples from clinical lung cancer patients. Conversely, a compensatory upregulation of USP24 activity resuced myeloma cells from apoptosis when USP9x activity was reduced via RNA-mediated knockdown or chemical inhibition with WP1130. Finally, USP24 is upregulated by DNA damaging agents and plays an important role in maintaining genome stability through its deubiquitination and stabilization of p53. This recombinant enzyme cleaves all poly-Ubiquitin chain linkages in vitro, and contains a c-terminal 10-His tag.

References

Peterson L.F. et al. (2015) Blood 125: 3588
Wang S.A. et al. (2017) Oncogene 36: 2930
Zhang L. & Gong F. (2016) Mol. Cell Oncol. 3: e1011888

Long Name

Ubiquitin Specific Protease 24

Entrez Gene IDs

23358 (Human); 329908 (Mouse); 313427 (Rat)

Alternate Names

Deubiquitinating enzyme 24; EC 3.4.19.12; FLJ31309; KIAA1057ubiquitin carboxyl-terminal hydrolase 24; ubiquitin specific peptidase 24; ubiquitin specific protease 24; ubiquitin thioesterase 24; Ubiquitin thiolesterase 24; ubiquitin-specific processing protease 24; Ubiquitin-specific-processing protease 24; USP24