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Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5b/UBE2D2 concentration of 0.1-1 μM.

Source

E. coli-derived human UbcH5b/UBE2D2 protein

Accession #

P62837

Predicted Molecular Mass

17 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-622

Formulation	Xmg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UbcH5b/UBE2D2

Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm (2). The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63 (4). Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively (5-7). Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules (8). Non-proteolytic ubiquitination of TRIM5-alphaby UbcH5b/UBE2D2 has been reported to block HIV reverse transcription (9). Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity (10). Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease (11).

References

Jensen, J.P. et al. (1995) J. Biol. Chem. 270:30408.
Umebayashi, K. et al. (2008) Mol. Biol. Cell 19:3454.
Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Ordureau, A. et al. (2008) Biochem. J. 409:43.
Chiang, M.H. et al. (2008) Biol. Reprod. 79:914.
Saville, M.K. et al. (2004) J. Biol. Chem. 279:42169.
Iyappan, S. et al. (2010) J. Biol. Chem. 285:33065.
Duncan, L.M. et al. (2006) EMBO J. 25:1635.
Yamauchi, K. et al. (2008) FEBS J. 275:1540.
Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
Hetzenecker, A.M. et al. (2012) Digestion 86:34.

Entrez Gene IDs

7322 (Human); 56550 (Mouse); 641452 (Rat)

Alternate Names

E2(17)KB2; EC 6.3.2.19; PUBC1; UBC4/5; UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); UBC5B; UBCH4; UbcH5b; UBE2D2; Ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D2

Related Research Areas

E2 Ubiquitin/Ubl Conjugating Enzymes
Search Ubiquitin-related Enzymes

Citations for Recombinant Human UbcH5b/UBE2D2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

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Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesisAuthors: YS Yoo, YJ Park, HS Lee, NTK Oanh, MY Cho, J Heo, ES Lee, H Cho, YY Park, H ChoCell Death Dis, 2019;10(12):938.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15Authors: TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D NijhawanCell Rep, 2019;29(6):1499-1510.e6.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
The E3 ubiquitin ligase Siah-1 suppresses avian reovirus infection by targeting p10 for degradationAuthors: X Chen, Z He, M Fu, Y Wang, H Wu, X Li, H Cao, SJ ZhengJ. Virol., 2018;0(0):.Applications: Bioassay
RNF126 Quenches RNF168 Function in the DNA Damage ResponseAuthors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X XuGenomics Proteomics Bioinformatics, 2018;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongationAuthors: N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T MaoProc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disabilityAuthors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van LoonSci Rep, 2017;7(1):15050.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress ResponseAuthors: ZQ Fan, JY Chen, JF Kuang, WJ Lu, W ShanFront Plant Sci, 2017;8(0):995.Applications: Bioassay
Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlying photomorphogenic development in ArabidopsisAuthors: X Xu, PK Kathare, VN Pham, Q Bu, A Nguyen, E HuqDevelopment, 2017;0(0):.Applications: Bioassay
BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundanceAuthors: B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O NilssonElife, 2017;6(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptorsAuthors: J Zhu, KY Lee, KA Jewett, HY Man, HJ Chung, NP TsaiPLoS Genet, 2017;13(2):e1006634.Species: HumanSample Types: Cell LysatesApplications: Bioassay

The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor TurnoverAuthors: R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T HoppeCell, 2017;169(3):470-482.e13.Applications: Bioassay

Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradationAuthors: X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H ZhangJ. Cell Biol., 2017;0(0):.Applications: Bioassay

Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death.Authors: Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, Han P, Oh YCell Death Differ, 2016;23(2):333-46.Species: N/ASample Types: ProteinApplications: Enzyme Assay

Identification and Characterization of MCM3 as a Novel KEAP1 SubstrateJ Biol Chem, 2016;0(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination

Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewalNat Commun, 2016;7(0):12326.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

14-3-3? loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-JunAuthors: Kumarkrishna RaychaudhuJ Biol Chem, 2016;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay

PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxiaAuthors: RS Banh, C Iorio, R Marcotte, Y Xu, D Cojocari, AA Rahman, J Pawling, W Zhang, A Sinha, CM Rose, M Isasa, S Zhang, R Wu, C Virtanen, T Hitomi, T Habu, SS Sidhu, A Koizumi, SE Wilkins, T Kislinger, SP Gygi, CJ Schofield, JW Dennis, BG Wouters, BG NeelNat. Cell Biol, 2016;18(7):803-13.Species: HumanSample Types: Cell LysatesApplications: Ubiquitination

Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillationAuthors: Francesca NazioJ. Cell Biol, 2016;215(6):841-856.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

Vaccinia-related kinase 2 mediates accumulation of polyglutamine aggregates via negative regulation of the chaperonin TRiC.Authors: Kim, Sangjune, Park, Do-Young, Lee, Dohyun, Kim, Wanil, Jeong, Young-Hu, Lee, Juhyun, Chung, Sung-Kee, Ha, Hyunjung, Choi, Bo-Hwa, Kim, Kyong-TaMol Cell Biol, 2014;34(4):643-52.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor.Authors: Smith, Christop, Berry, Donna M, McGlade, C JaneJ Cell Sci, 2013;126(0):1366-80.Species: N/ASample Types: ProteinApplications: Bioassay

Characterization of ML-IAP protein stability and physiological role in vivo.Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, DomagojBiochem J, 2012;447(3):427-36.Species: HumanSample Types: ProteinApplications: Bioassay

DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylationregulates p53 transcriptional activation.Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow AJ Biol Chem, 2012;287(36):30789-99.Species: N/ASample Types: ProteinApplications: Bioassay

The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.Authors: Lu, Yunzhe, Li, Jiezhi, Cheng, Dongmei, Parameswaran, Balaji, Zhang, Shaohua, Jiang, Zefei, Yew, P Renee, Peng, Junmin, Ye, Qinong, Hu, YanfenJ Biol Chem, 2012;287(49):41014-22.Species: N/ASample Types: Recombinant ProteinApplications: Ubiquitination

HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.Authors: Sosin, Angela M, Burger, Angelika, Siddiqi, Aisha, Abrams, Judith, Mohammad, Ramzi M, Al-Katib, Ayad MJ Hematol Oncol, 2012;5(0):57.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234.Authors: Xin DW, Liao S, Xie ZPPLoS Pathog., 2012;8(5):e1002707.