Recombinant Human LC3A Rhodamine 110 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-455
| Formulation | 0.62 mg/ml (44 µM) in 50 mM MES, pH 6.0, 100 mM NaCl, 20% glycerol |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: LC3A
Microtubule-associated proteins 1A/1B light chain 3A (also known as MAP1LC3A) is an Ubiquitin-like modifier of the ATG8 family and is involved in the formation of the autophagosomes. This protein interacts directly with Sequestosome-1 and is recruited to inclusion bodies containing polyubiquitinated protein aggregates where it plays a role in inclusion body degradation by autophagy. MAP1LC3A-Rhodamine110 (Rh110) is a fluorogenic substrate for the autophagy hydrolase ATG4B. This substrate is useful for studying ATG4B when detection sensitivity or continuous monitoring of activity is essential.
- Borodovsky A., et al. (2002) Chem. Biol. 9:1149-1159
- Hemelaar J., et al. (2003) J Biol. Chem. 278:51841-51850
- Kessler B.M. (2006) Exp. Rev. Proteomics. 3:213-221
- Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618
- Love K.R, et al. (2007) Nat. Chem. Biol. 3:697-705
- Wilkinson K.D. et al. (2005) Meth. Enz. 399:37-51
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