AQUApure Tetra-Ub Chains (K48-linked) Protein, CF Summary
Ub-AQUA analysis:
K48: 99.31%
K11: 0.36%
K63:0.19%
K6 : 0.09%
All other linkages ≤ 0.03%
Ubiquitin chains vary in length, linkage, and function. K48-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="">
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-210B
| Formulation | 1 mg/ml (29 μM) in sterile, deionized water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tetra-Ubiquitin
Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF"s and/or other Ubiquitin-sensing elements.
K48-linked Tetra-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.
- Kirkpatrick D.S., et al. (2006) Nat Cell Biol. 8(7): 700-10
- Ordureau, A., et al. (2014) Mol. Cell 56(3): 360–375
- Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
- Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756
Citations for AQUApure Tetra-Ub Chains (K48-linked) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- The ubiquitin interacting motifs of USP37 act on the proximal Ub of a di-Ub chain to enhance catalytic efficiencyAuthors: N Manczyk, G Veggiani, J Teyra, AW Strilchuk, SS Sidhu, F SicheriSci Rep, 2019;9(1):4119.Applications: Bioassay
- Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunityAuthors: NW Zammit, OM Siggs, PE Gray, K Horikawa, DB Langley, SN Walters, SR Daley, C Loetsch, J Warren, JY Yap, D Cultrone, A Russell, EK Malle, JE Villanueva, MJ Cowley, V Gayevskiy, ME Dinger, R Brink, D Zahra, G Chaudhri, G Karupiah, B Whittle, C Roots, E Bertram, M Yamada, Y Jeelall, A Enders, BE Clifton, PD Mabbitt, CJ Jackson, SR Watson, CN Jenne, LL Lanier, T Wiltshire, MH Spitzer, GP Nolan, F Schmitz, A Aderem, BT Porebski, AM Buckle, DW Abbott, JB Ziegler, ME Craig, P Benitez-Ag, J Teo, SG Tangye, C King, M Wong, MP Cox, W Phung, J Tang, W Sandoval, IE Wertz, D Christ, CC Goodnow, ST GreyNat. Immunol., 2019;20(10):1299-1310.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilizationAuthors: S Yang, L Liu, C Cao, N Song, Y Wang, S Ma, Q Zhang, N Yu, X Ding, F Yang, S Tian, K Zhang, T Sun, J Yang, Z Yao, S Wu, L ShiNat Commun, 2018;9(1):1285.Applications: Bioassay
- The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant ImmunityAuthors: T Schultz-La, A Lenk, K Kalinowska, LK Vestergaar, C Pedersen, E Isono, H Thordal-ChCell Rep, 2018;25(9):2329-2338.e5.Species: Plant - Arabidopsis thalianaSample Types: Recombinant ProteinApplications: Ubiquitination
- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- OTUD4 Is a Phospho-Activated K63 Deubiquitinase that Regulates MyD88-Dependent SignalingAuthors: Y Zhao, MC Mudge, JM Soll, RB Rodrigues, AK Byrum, EA Schwarzkop, TR Bradstreet, SP Gygi, BT Edelson, N MosammaparMol. Cell, 2018;69(3):505-516.e5.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yieldAuthors: S Wang, K Wu, Q Qian, Q Liu, Q Li, Y Pan, Y Ye, X Liu, J Wang, J Zhang, S Li, Y Wu, X FuCell Res., 2017;27(9):1142-1156.Species: Plant - Oryza sativa (Rice)Sample Types: Recombinant ProteinApplications: Bioassay
- Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteasesAuthors: L Lauinger, J Li, A Shostak, IA Cemel, N Ha, Y Zhang, PE Merkl, S Obermeyer, N Stankovic-, T Schafmeier, WJ Wever, AA Bowers, KP Carter, AE Palmer, H Tschochner, F Melchior, RJ Deshaies, M Brunner, A DiernfellnNat. Chem. Biol., 2017;0(0):.
- Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targetsAuthors: X Lu, U Nowicka, V Sridharan, F Liu, L Randles, D Hymel, M Dyba, SG Tarasov, NI Tarasova, XZ Zhao, J Hamazaki, S Murata, TR Burke, KJ WaltersNat Commun, 2017;8(0):15540.Species: N/ASample Types: ProteinApplications: Bioassay
- Tandem UIMs confer Lys48 ubiquitin chain substrate preference to deubiquitinase USP25Authors: K Kawaguchi, K Uo, T Tanaka, M KomadaSci Rep, 2017;7(0):45037.Species: N/ASample Types: ProteinApplications: Bioassay
- In�Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the ProteasomeAuthors: H Tsuchiya, F Ohtake, N Arai, A Kaiho, S Yasuda, K Tanaka, Y SaekiMol. Cell, 2017;66(4):488-502.e7.Applications: Bioassay
- The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as afeedback regulator of biochemical and oncogenic activity.Authors: Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh BJ Biol Chem, 2015;290(1):296-309.Species: N/ASample Types: ProteinApplications: Binding Assay
- Non-covalent interaction between polyubiquitin and GTP cyclohydrolase 1 dictates its degradation.Authors: Zhao, Yu, Zhu, Huaiping, Zou, Ming-HuiPLoS ONE, 2012;7(9):e43306.Species: N/ASample Types: ProteinApplications: Bioassay
- Differential polyubiquitin recognition by tandem ubiquitin binding domains ofRabex-5.Authors: Shin D, Lee S, Han S, Ren S, Kim S, Aikawa Y, Lee SBiochem Biophys Res Commun, 2012;423(4):757-62.Species: N/ASample Types: ProteinApplications: Bioassay
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