Recombinant Human His6-UBE2E3 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-678
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2E3
Ubiquitin-conjugating Enzyme E2E 3 (UBE2E3), also known as Ubiquitin-conjugating Enzyme H9 (UbcH9), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UBE2E3 has a predicted molecular weight of 23 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2E3 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). This enzyme appears to be localized in the nucleus of interphase cells and cell-wide in mitotic cells (3). UBE2E3 may be regulated at the post-transcriptional level by miR-143 (4). The auto-ubiquitination of the ARA54, RNF8, and bacterially-encoded NleG Ubiquitin ligases (E3s) is mediated by UBE2E3 (5,6). UBE2E3 also functions with NEDD4L to regulate ENaC activity (7). Physiologically, UBE2E3 is required for the proliferation of retinal pigment epithelial cells via the negative regulation of p27/Kip1 levels (3). UBE2E3 may also be required for HIV-1 replication (8).This protein has an N-terminal His6-tag. (Accession # NP_006348.1)
- Ito, K. et al. (1999) Cytogenet. Cell Genet. 84:99.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Plafker, K.S. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5611.
- Trakooljul, N. et al. (2010) Anim. Genet. 41:357.
- Ito, K. et al. (2001) Eur. J. Biochem. 268:2725.
- Wu, B. et al. (2010) PLoS Pathog. 6:e1000960.
- Debonneville, C. et al. (2004) Mol. Cell. Biol. 24:2397.
- Dziuba, N. et al. (2012) AIDS Res. Hum. Retroviruses 28:1329.
Citation for Recombinant Human His6-UBE2E3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- A Virally Encoded DeSUMOylase Activity Is Required for Cytomegalovirus Reactivation from LatencyAuthors: EL Poole, VG Kew, JCH Lau, MJ Murray, T Stamminger, JH Sinclair, MB ReevesCell Rep, 2018;24(3):594-606.Applications: Bioassay
FAQs
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