Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF Summary
Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within thealpha 1 andalpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).
This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.
Specifications
- 12 months from date of receipt, 2 to 8 °C as supplied.
- 3 months, 2 to 8 °C under sterile conditions after opening.
Product Datasheets
Background: HR23A/Rad23A TUBE1
- Chen, L. et al. (2001) EMBO Rep. 2:933.
- Wang, Q. et al. (2003) Biochemistry 42:13529.
- Hjerpe, R. et al. (2009) EMBO Rep. 10:1250.
- Hurley, J.H. et al. (2006) Biochem. J. 399:361.
- Lopitz-Otsoa, F. et al. (2010) Biochem. Soc. Trans. 38:40.
Reagents Provided
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF
There are currently no reviews for this product. Be the first toreview Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF and earn rewards!
Have you used Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
