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Recombinant SARS-CoV-2 3CL Protease is a cysteine protease that cleaves proteins with sequences including LQ[S/A/G). The cleavage occurs c-terminal to the glutamine residue. Reaction conditions will need to be optimized for each specific application.

Source

E. coli-derived sars-cov-2 3CL Protease proteinSer3264 - Gln3569

Accession #

P0DTC1

Predicted Molecular Mass

34 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-720

Formulation	1.7 mg/ml (50 μM) in 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 100 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Data Images

Inhibition Activity

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GC376 achieves 50% inhibition of 30 nM SARS-CoV-2 3CL Protease (E-720) at a concentation of 54-71 nM.

SDS-PAGE

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SDS-PAGE of Recombinant SARS-CoV-2 3CL Protease. 1 μg and 2 μg of Recombinant SARS-CoV-2 3CL Protease (Catalog # E-720) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 34 kDa.

Mass Spectrometry LC-MS analysis of SARS-CoV-2 3CL Protease

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LC-MS analysis of SARS-CoV-2 3CL Protease. SARS-CoV-2 3CL Protease (Catalog # E-720) was analyzed by a Thermo Q Exactive HF instrument then deconvoluted using Thermo BioPharma Finder software. The expected monoisotopic mass is 33774.478 Da.

Reconstitution Calculator

Background: 3CL Protease

The 3CL protease (aka 3CL^pro, M^pro or "Main" Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.3CL^proactivity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid. Along with the CoV-2 Papain-Like Protease (catalog number E-611),3CL^propresents an attractive target for therapeutic intervention for COVID-19. Because no human proteases with a similar cleavage specificity are known, inhibitors of3CL^proare unlikely to cause mechanism-based toxicity. This recombinant protein is untagged.

References