Recombinant Human His6-UBE2R1/CDC34 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-610
| Formulation | X mg/ml (μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mMTCEP, 10% Glycerol (v/v) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2R1/CDC34
Ubiquitin-conjugating Enzyme H3 (UbcH3), also known as Cell Division Cycle 34 (Cdc34), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UbcH3 has a predicted molecular weight of 26 kDa. The human protein shares 98% amino acid (aa) sequence identity with the mouse and rat orthologs. In addition to an E2 catalytic core domain, UbcH3 has two Ubiquitin binding sites, UBS1, from aa 205-215, and UBS2, from aa 216-225, and a C-terminal acidic tail domain (2,3). UbcH3 is required for efficient cell cycle progression in human cells (4). UbcH3 interacts with the SCF(Fbw7) and SCF(Skp2) Ubiquitin ligases (E3s) to target the key cell cycle proteins Myc and p27/Kip1, respectively, for ubiquitination and degradation (4,5). Furthermore, CK2 has been shown to phosphorylate human UbcH3 on Ser203, Ser222, and Ser231 in cycling cells (6). The proliferation of cancer cell lines is inhibited following treatment with a human UbcH3 inhibitor, suggesting that UbcH3 also plays a significant role in cancer (7). This protein has an N-terminal His6-tag.
- Plon, S.E. et al. (1993) Proc. Natl. Acad. Sci. USA 90:10484.
- Choi, Y.S. et al. (2010) J. Biol. Chem. 285:17754.
- Block, K. et al. (2005) Cell Cycle 4:1421.
- Butz, N. et al. (2005) Exp. Cell Res. 303:482.
- Popov, N. et al. (2010) Nat. Cell Biol. 12:973.
- Sadowski, M. et al. (2007) Biochem. J. 405:569.
- Ceccarelli, D.F. et al. (2011) Cell 145:1075.
Citations for Recombinant Human His6-UBE2R1/CDC34 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing FactorsAuthors: TR de Moura, S Mozaffari-, CZK Szabó, J Schmitzová, O Dybkov, C Cretu, M Kachala, D Svergun, H Urlaub, R Lührmann, V PenaMol. Cell, 2018;69(6):979-992.e6.Species: BacteriaSample Types: Cell LysatesApplications: Bioassay
- BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformationAuthors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M CarboneNature, 2017;546(7659):549-553.Species: N/ASample Types: ProteinApplications: Bioassay
- SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S E2s to ubiquitylate ?-TrCP1 via K11-linkage for degradationSci Rep, 2016;6(0):37441.Species: HumanSample Types: Whole CellsApplications: Bioassay
- A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay LJ Biol Chem, 2012;287(52):43410-6.Species: HumanSample Types: Recombinant ProteinApplications: Ubiquitination
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