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Recombinant Human His6-Use1/UBE2Z is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Use1/UBE2Z concentration of 0.1-1 μM.

Source

E. coli-derived human Use1/UBE2Z proteinContains an N-terminal 6-His tag

Accession #

Q9H832

Predicted Molecular Mass

41 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-677

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v),1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Use1/UBE2Z

Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis (1). Use1/UBE2Z has a predicted molecular weight of 38 kDa. The human enzyme shares 98% amino acid sequence identity with its mouse and rat orthologs. It has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). Use1/UBE2Z functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation (3). Use1/UBE2Z expression is enriched in the cytoplasm where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway (4). Use1/UBE2Z also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma (5). This protein has an N-terminal His₆-tag.

References

Gu, X. et al. (2007) Mol. Biol. Rep. 34:183.
Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Jin, J. et al. (2007) Nature 447:1135.
Lee, P.C. et al. (2011) Mol. Cell 43:392.
Aichem, A. et al. (2010) Nat. Commun. 4:13.

Entrez Gene IDs

65264 (Human); 268470 (Mouse)

Alternate Names

MDS032; p31; protein p31; putative MAPK activating protein PM26; Putative MAPK-activating protein PM26; Q-SNARE; SLT1; SNARE-like tail-anchored protein 1 homolog; UBE2Z; unconventional SNARE in the ER 1 homolog (S. cerevisiae); Use1; USE1L; USE1-like protein; vesicle transport protein USE1