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Recombinant human USP22 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP22 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) substrates. Note: Recombinant USP22 is not active toward poly-Ubiquitin chains, Ubiquitinated histones and other Ubiquitinated protein substrates in the absence of adapter proteins such as ATXN7L or ENY2.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP22 proteinContains an N-terminal 6-His tag

Accession #

Q9UPT9

Predicted Molecular Mass

59 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-608

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, and 2 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP22

Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a specialized cysteine protease with a predicted molecular weight of 60 kDa.USP22 is a member of the peptidase C19 family (UBP8 subfamily) and the human protein shares 98% amino acid sequence identity with its mouse ortholog.USP22 is a member of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a 2 MDa protein machine that mediates the acetylation and deubiquitination of histones as well as non-histone substrates.Within SAGA, USP22 binds directly with ATXN7L3 and provides the activity required to deubiquitinate mono-Ubiquitinated histone H2B.Recent findings suggest USP27X and USP51, which function independently of SAGA, may compete with USP22 for ATXN7L3 and ENY2 binding, and that imbalances in these activities may contribute to human diseases including cancer.

References

Atanassov B.S. et al. (2016) Mol. Cell 62:558.
Atanassov B.S. et al. (2011) FEBS Lett. 585:2016.
Atanassov B.S. & Dent S.R. (2016) personal communication.
Koutelou E. et al. (2010) Curr Opin Cell Biol. 22:374.
Lang G. et al. (2011) Mol Cell Biol. 31:3734.
Zhang X.Y. et al. (2008) Mol. Cell 29:102.
Zhao Y. et al. (2008) Mol. Cell 29:92.

Long Name

Ubiquitin Specific Protease 22

Entrez Gene IDs

23326 (Human); 216825 (Mouse); 303201 (Rat)

Alternate Names

Deubiquitinating enzyme 22; EC 3.1.2.15; EC 3.4.19.12; KIAA1063ubiquitin carboxyl-terminal hydrolase 22; KIAA1064; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin Thioesterase 22; Ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; USP22; USP3L