Recombinant Human GST-AMSH-LP Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-551
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: AMSH-LP
AMSH-like protease (AMSH-LP, also known as STAM-Binding Protein-Like 1, STAMBPL1) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). 436 amino acids in length, AMSH-LP is 56% identical and 73% similar to AMSH, an enzyme that functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. Like AMSH, AMSH-LP only cleaves K63-linked poly-Ubiquitin chains in vitro; however, unlike AMSH, AMSH-LP does not appear to associate with STAM protein. This recombinant protein is full-length and contains an N-terminal GST tag.
- Davies, C.W. et al. (2011) J. Mol. Biol. 413: 416
- Sato, Y. et al. (2008) Nature 455: 358
FAQs
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