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Reaction conditions will need to be optimized for each specific application. We recommend an initialHis6-USP10concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555).

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP10 proteinContains an C-terminal 6-His tag

Accession #

Q14694

Predicted Molecular Mass

88 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-592

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP10

Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a specialized cysteine protease with a predicted molecular weight of 87 kDa. USP10 is a member of the peptidase C19 family and the human protein shares 83% amino acid sequence identity with its mouse ortholog. Reported USP10 targets include p53, BECN1, SNX3 and CFTR. USP10 translocates to the nucleus in response to DNA damage and deubiquitinates p53, thus regulating the p53-dependent DNA damage response. USP10 deubiquitination of BECN1 leads to stabilization of PIK3C3/VPS34-containing complexes which drives autophagy. The small molecule Spautin-1 exerts its potent inhibitory effects on autophagy partially through the inhibition of USP10 activity.

References

Boulkroun S., et al. (2008) Am. J. Physiol. 295:F889-F900
Liu J., et al. (2011) Cell 147: 223
Yuan J., et al. (2010) Cell 140: 384

Long Name

Ubiquitin Specific Protease 10

Entrez Gene IDs

9100 (Human); 22224 (Mouse); 307905 (Rat)

Alternate Names

Deubiquitinating enzyme 10; EC 3.4.19.12; KIAA0190MGC2621; ubiquitin carboxyl-terminal hydrolase 10; ubiquitin specific peptidase 10; ubiquitin specific protease 10; ubiquitin thioesterase 10; Ubiquitin Thiolesterase 10; Ubiquitin-specific-processing protease 10; UBPO; Uchrp; USP10