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蚂蚁淘在线 / 品牌中心 / Biorbyt / Biorbyt/Rat CD133 Human Cells Transfected Lysate (positive control) (denatured)/orb174679
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Rat CD133 Human Cells Transfected Lysate (positive control) (denatured)
Catalog Number: orb174679
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DatasheetProduct Overview
| Product Name | Rat CD133 Human Cells Transfected Lysate (positive control) (denatured) |
|---|---|
| Catalog Number | orb174679 |
| Hazard Information | Non-Toxic |
| Target | Rat CD133 |
Product Properties
| Form/Appearance | 50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue |
|---|---|
| Storage | Store at -80°C. Aliquot to avoid repeated freezing and thawing |
| Note | For research use only. |
| Biological Origin | Human Cells |
Product Description
Human Cells transfected lysate in which Rat CD133/PROM1/Prominin 1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Application Notes
| Application Notes | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol |
|---|
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Biorbyt的mCherry。在cds质粒转染的293HEK细胞中，通过Western blot检测到一条29 kDa的条带。该抗体可很好地识别tdTomato，不识别GFP（绿色荧光蛋白）。
